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  • Alternative name

    Aromatase ELISA KIT; CYPXIX ELISA KIT; Cytochrome P-450AROM ELISA KIT; Cytochrome P450 19A1 ELISA KIT; Estrogen synthaseCYP19A1 ELISA KIT; ARO1 ELISA KIT; CYAR ELISA KIT; CYP19 ELISA KIT

  • Catalog
  • species
  • GeneARO
  • Standard CurveHuman ARO ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Aromatase (ARO). No significant cross-reactivity or interference between Aromatase (ARO) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman ARO ELISA Kit allows for the in vitro quantitative determination of ARO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase (ARO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aromatase (ARO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase (ARO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aromatase (ARO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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