Human HLA-DRB5 ELISA Kit

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  • Alternative name

    Human DR beta-5 ELISA Kit;Human DR2-beta-2 ELISA Kit;Human Dw2 ELISA Kit;Human MHC class II antigen DRB5 ELISA Kit;Human major histocompatibility complex, class II, DR beta 5 ELISA Kit;Human HLA class II histocompatibility antigen, DR-5 beta chain ELISA Kit;

  • Catalog
    E018855
  • species
    Human
  • GeneHLA-DRB5
  • Standard CurveHuman HLA-DRB5 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Human DRB5. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman HLA-DRB5 ELISA Kit allows for the in vitro quantitative determination of HLA-DRB5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human hla class ii histocompatibility antigen, dr beta 5 chain,DRB5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DRB5, During the reaction, DRB5 in the sample or standard competes with a fixed amount of biotin-labeled DRB5 for sites on a pre-coated Monoclonal antibody specific to DRB5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DRB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human HLA class II histocompatibility antigen, DR beta 5 chain Protein information
  • Uniprot ID DRB5_HUMAN
  • Uniprot AC Q9MY54; Q9XRX6;
  • UniGene Hs.485130; Hs.534322; Hs.696211; Hs.736560;
  • GeneID 3127
  • KEGG hsa:3127;
  • Human HLA class II histocompatibility antigen, DR beta 5 chain Protein SEQUENCE
  • SEQUENCE 266 AA; 30056 MW; 0D4335BAEEA6AF22 CRC64;

    MVCLKLPGGS YMAKLTVTLM VLSSPLALAG DTRPRFLQQD KYECHFFNGT

    ERVRFLHRDI YNQEEDLRFD SDVGEYRAVT ELGRPDAEYW NSQKDFLEDR

    RAAVDTYCRH NYGVGESFTV QRRVEPKVTV YPARTQTLQH HNLLVCSVNG

    FYPGSIEVRW FRNSQEEKAG VVSTGLIQNG DWTFQTLVML ETVPRSGEVY

    TCQVEHPSVT SPLTVEWRAQ SESAQSKMLS GVGGFVLGLL FLGAGLFIYF

    KNQKGHSGLH PTGLVS

  • UCSC uc003obj.4; human.;



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