Human HLA-DRB5 ELISA Kit

Alternative name
Human DR beta-5 ELISA Kit;Human DR2-beta-2 ELISA Kit;Human Dw2 ELISA Kit;Human MHC class II antigen DRB5 ELISA Kit;Human major histocompatibility complex, class II, DR beta 5 ELISA Kit;Human HLA class II histocompatibility antigen, DR-5 beta chain ELISA Kit;
Catalog
E018855
species
Human
GeneHLA-DRB5
SpecificityThis assay recognizes recombinant and natural Human DRB5. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman HLA-DRB5 ELISA Kit allows for the in vitro quantitative determination of HLA-DRB5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination of human hla class ii histocompatibility antigen, dr beta 5 chain,DRB5 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DRB5, During the reaction, DRB5 in the sample or standard competes with a fixed amount of biotin-labeled DRB5 for sites on a pre-coated Monoclonal antibody specific to DRB5. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DRB5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human HLA class II histocompatibility antigen, DR beta 5 chain Protein information
Uniprot ID DRB5_HUMAN
Uniprot AC Q9MY54; Q9XRX6;
UniGene Hs.485130; Hs.534322; Hs.696211; Hs.736560;
GeneID 3127
KEGG hsa:3127;
Human HLA class II histocompatibility antigen, DR beta 5 chain Protein SEQUENCE
SEQUENCE 266 AA; 30056 MW; 0D4335BAEEA6AF22 CRC64;
MVCLKLPGGS YMAKLTVTLM VLSSPLALAG DTRPRFLQQD KYECHFFNGT
ERVRFLHRDI YNQEEDLRFD SDVGEYRAVT ELGRPDAEYW NSQKDFLEDR
RAAVDTYCRH NYGVGESFTV QRRVEPKVTV YPARTQTLQH HNLLVCSVNG
FYPGSIEVRW FRNSQEEKAG VVSTGLIQNG DWTFQTLVML ETVPRSGEVY
TCQVEHPSVT SPLTVEWRAQ SESAQSKMLS GVGGFVLGLL FLGAGLFIYF
KNQKGHSGLH PTGLVS
UCSC uc003obj.4; human.;

Category

Human HLA-DRB5 ELISA Kit

Human DR beta-5 ELISA Kit;Human DR2-beta-2 ELISA Kit;Human Dw2 ELISA Kit;Human MHC class II antigen DRB5 ELISA Kit;Human major histocompatibility complex, class II, DR beta 5 ELISA Kit;Human HLA class II histocompatibility antigen, DR-5 beta chain ELISA Kit;