Mouse Scnn1a ELISA Kit

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  • Alternative name

    Mouse alpha-NaCH ELISA Kit;Mouse epithelial Na(+) channel subunit alpha ELISA Kit;Mouse alpha-ENaC ELISA Kit;Mouse nonvoltage-gated sodium channel 1 subunit alpha ELISA Kit;Mouse SCNEA ELISA Kit;Mouse ENaC ELISA Kit;Mouse Scnn1 ELISA Kit;Mouse mENaC ELISA Kit;Mouse sodium channel, nonvoltage-gated 1 alpha ELISA Kit;Mouse amiloride-sensitive sodium channel subunit alpha ELISA Kit;Mouse ENaC alpha ELISA Kit;Mouse amiloride-sensitive epithelial sodium channel ELISA Kit;Mouse epithelial sodium channel alpha subunit ELISA Kit;Mouse sodium channel, nonvoltage-gated, type I, alpha polypeptide ELISA Kit;

  • Catalog
    E043598
  • species
    Mouse
  • GeneScnn1a
  • Standard CurveMouse Scnn1a ELISA Kit
  • Other Species Human SCNN1A ELISA KitBovine SCNN1A ELISA KitGuinea Pig SCNN1A ELISA KitChicken SCNN1A ELISA KitRabbit SCNN1A ELISA KitRat Scnn1a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SCNN1A. No significant cross-reactivity or interference between SCNN1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCNN1A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Scnn1a ELISA Kit allows for the in vitro quantitative determination of Scnn1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This SCNN1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SCNN1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: SCNN1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SCNN1A antibody and an SCNN1A-HRP conjugate. The assay sample and buffer are incubated together with SCNN1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCNN1A concentration since SCNN1A from samples and SCNN1A-HRP conjugate compete for the anti-SCNN1A antibody binding site. Since the number of sites is limited, as more sites are occupied by SCNN1A from the sample, fewer sites are left to bind SCNN1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCNN1A concentration in each sample is interpolated from this standard curve.
  • Mouse Amiloride-sensitive sodium channel subunit alpha Protein information
  • Uniprot ID SCNNA_MOUSE
  • Uniprot AC Q61180; Q9WU37;
  • UniGene Mm.144114;
  • GeneID 20276
  • KEGG mmu:20276;
  • Mouse Amiloride-sensitive sodium channel subunit alpha Protein SEQUENCE
  • SEQUENCE 699 AA; 78893 MW; 5B083BE8769B017A CRC64;

    MLDHTRAPEL NLDLDLDVSN SPKGSMKGNN FKEQDLCPPL PMQGLGKGDK

    REEQALGPEP SEPRQPTEEE EALIEFHRSY RELFQFFCNN TTIHGAIRLV

    CSKHNRMKTA FWAVLWLCTF GMMYWQFALL FEEYFSYPVS LNINLNSDKL

    VFPAVTVCTL NPYRYTEIKE DLEELDRITE QTLFDLYKYN SSYTRQAGGR

    RRSTRDLRGA LPHPLQRLRT PPPPNPARSA RSASSSVRDN NPQVDRKDWK

    IGFQLCNQNK SDCFYQTYSS GVDAVREWYR FHYINILSRL PDTSPALEEE

    ALGSFIFTCR FNQAPCNQAN YSQFHHPMYG NCYTFNNKNN SNLWMSSMPG

    VNNGLSLTLR TEQNDFIPLL STVTGARVMV HGQDEPAFMD DGGFNVRPGV

    ETSISMRKEA LDSLGGNYGD CTENGSDVPV KNLYPSKYTQ QVCIHSCFQE

    NMIKKCGCAY IFYPKPKGVE FCDYLKQSSW GYCYYKLQAA FSLDSLGCFS

    KCRKPCSVTN YKLSAGYSRW PSVKSQDWIF EMLSLQNNYT INNKRNGVAK

    LNIFFKELNY KTNSESPSVT MVSLLSNLGS QWSLWFGSSV LSVVEMAELI

    FDLLVITLIM LLHRFRSRYW SPGRGARGAR EVASTPASSF PSRFCPHPTS

    PPPSLPQQGT TPPLALTAPP PAYATLGPSA SPLDSAVPGS SACAPAMAL



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