Mouse Scnn1a ELISA Kit

Alternative name
Mouse alpha-NaCH ELISA Kit;Mouse epithelial Na(+) channel subunit alpha ELISA Kit;Mouse alpha-ENaC ELISA Kit;Mouse nonvoltage-gated sodium channel 1 subunit alpha ELISA Kit;Mouse SCNEA ELISA Kit;Mouse ENaC ELISA Kit;Mouse Scnn1 ELISA Kit;Mouse mENaC ELISA Kit;Mouse sodium channel, nonvoltage-gated 1 alpha ELISA Kit;Mouse amiloride-sensitive sodium channel subunit alpha ELISA Kit;Mouse ENaC alpha ELISA Kit;Mouse amiloride-sensitive epithelial sodium channel ELISA Kit;Mouse epithelial sodium channel alpha subunit ELISA Kit;Mouse sodium channel, nonvoltage-gated, type I, alpha polypeptide ELISA Kit;
Catalog
E043598
species
Mouse
GeneScnn1a
SpecificityThis assay has high sensitivity and excellent specificity for detection of SCNN1A. No significant cross-reactivity or interference between SCNN1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SCNN1A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Scnn1a ELISA Kit allows for the in vitro quantitative determination of Scnn1a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalIntended Uses: This SCNN1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SCNN1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: SCNN1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SCNN1A antibody and an SCNN1A-HRP conjugate. The assay sample and buffer are incubated together with SCNN1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCNN1A concentration since SCNN1A from samples and SCNN1A-HRP conjugate compete for the anti-SCNN1A antibody binding site. Since the number of sites is limited, as more sites are occupied by SCNN1A from the sample, fewer sites are left to bind SCNN1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCNN1A concentration in each sample is interpolated from this standard curve.
Mouse Amiloride-sensitive sodium channel subunit alpha Protein information
Uniprot ID SCNNA_MOUSE
Uniprot AC Q61180; Q9WU37;
UniGene Mm.144114;
GeneID 20276
KEGG mmu:20276;
Mouse Amiloride-sensitive sodium channel subunit alpha Protein SEQUENCE
SEQUENCE 699 AA; 78893 MW; 5B083BE8769B017A CRC64;
MLDHTRAPEL NLDLDLDVSN SPKGSMKGNN FKEQDLCPPL PMQGLGKGDK
REEQALGPEP SEPRQPTEEE EALIEFHRSY RELFQFFCNN TTIHGAIRLV
CSKHNRMKTA FWAVLWLCTF GMMYWQFALL FEEYFSYPVS LNINLNSDKL
VFPAVTVCTL NPYRYTEIKE DLEELDRITE QTLFDLYKYN SSYTRQAGGR
RRSTRDLRGA LPHPLQRLRT PPPPNPARSA RSASSSVRDN NPQVDRKDWK
IGFQLCNQNK SDCFYQTYSS GVDAVREWYR FHYINILSRL PDTSPALEEE
ALGSFIFTCR FNQAPCNQAN YSQFHHPMYG NCYTFNNKNN SNLWMSSMPG
VNNGLSLTLR TEQNDFIPLL STVTGARVMV HGQDEPAFMD DGGFNVRPGV
ETSISMRKEA LDSLGGNYGD CTENGSDVPV KNLYPSKYTQ QVCIHSCFQE
NMIKKCGCAY IFYPKPKGVE FCDYLKQSSW GYCYYKLQAA FSLDSLGCFS
KCRKPCSVTN YKLSAGYSRW PSVKSQDWIF EMLSLQNNYT INNKRNGVAK
LNIFFKELNY KTNSESPSVT MVSLLSNLGS QWSLWFGSSV LSVVEMAELI
FDLLVITLIM LLHRFRSRYW SPGRGARGAR EVASTPASSF PSRFCPHPTS
PPPSLPQQGT TPPLALTAPP PAYATLGPSA SPLDSAVPGS SACAPAMAL

Category

Mouse Scnn1a ELISA Kit