Angiotensinogen ELISA KIT; Serpin A8Cleaved into the following 8 chains:Angiotensin-1 ELISA KIT; Alternative name(s): ELISA KIT; Angiotensin 1-10 ELISA KIT; Angiotensin I ELISA KIT; Ang IAngiotensin-2 ELISA KIT; Alternative name(s): ELISA KIT; Angiotensin 1-8 ELISA KIT; Angiotensin II ELISA KIT; Ang IIAngiotensin-3 ELISA KIT; Alternative name(s): ELISA KIT; Angiotensin 2-8 ELISA KIT; Angiotensin III ELISA KIT; Ang III ELISA KIT; Des-Asp-angiotensin IIAngiotensin-4 ELISA KIT; Alternative name(s): ELISA KIT; Angiotensin 3-8 ELISA KIT; Angiotensin IVAGT ELISA KIT; SERPINA8 ELISA KIT; Ang I ELISA KIT; Ang II ELISA KIT; Ang III ELISA KIT; Ang IV ELISA KIT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Angiotensin I (AngI).
No significant cross-reactivity or interference between Angiotensin I (AngI) and analogues was observed.
Intended UseMouse AngI ELISA Kit allows for the in vitro quantitative determination of AngI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I (AngI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I (AngI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I (AngI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiotensin I (AngI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.