Human OSM ELISA Kit

Alternative name
Human OSM ELISA Kit;Human oncostatin M ELISA Kit;Human oncostatin-M ELISA Kit;
Catalog
E027690
species
Human
GeneOSM
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman OSM ELISA Kit allows for the in vitro quantitative determination of OSM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: OSM ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OSM. Standards or samples are then added to the microtiter plate wells and OSM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OSM present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for OSM are added to each well to "sandwich" the OSM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OSM and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSM concentration in each sample is interpolated from this standard curve.
Human Oncostatin-M Protein information
Uniprot ID ONCM_HUMAN
Uniprot AC P13725; Q6FHP8; Q9UCP6;
UniGene Hs.248156;
GeneID 5008
KEGG hsa:5008;
Human Oncostatin-M Protein SEQUENCE
SEQUENCE 252 AA; 28484 MW; A5BE281175D101B9 CRC64;
MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ
DTSRLLDPYI RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN
ATLGCVLHRL ADLEQRLPKA QDLERSGLNI EDLEKLQMAR PNILGLRNNI
YCMAQLLDNS DTAEPTKAGR GASQPPTPTP ASDAFQRKLE GCRFLHGYHR
FMHSVGRVFS KWGESPNRSR RHSPHQALRK GVRRTRPSRK GKRLMTRGQL
PR
UCSC uc003ahb.4; human.;

Category

Human OSM ELISA Kit

Human OSM ELISA Kit;Human oncostatin M ELISA Kit;Human oncostatin-M ELISA Kit;