Human FECH ELISA Kit

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  • Alternative name

    Human heme synthase ELISA Kit;Human protoheme ferro-lyase ELISA Kit;Human EPP ELISA Kit;Human FCE ELISA Kit;Human ferrochelatase ELISA Kit;Human ferrochelatase, mitochondrial ELISA Kit;Human heme synthetase ELISA Kit;Human protoporphyria ELISA Kit;

  • Catalog
    E014723
  • species
    Human
  • GeneFECH
  • Standard CurveHuman FECH ELISA Kit
  • Other Species Mouse Fech ELISA KitBovine FECH ELISA KitChicken FECH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ferrochelatase (FECH). No significant cross-reactivity or interference between Ferrochelatase (FECH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman FECH ELISA Kit allows for the in vitro quantitative determination of FECH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferrochelatase (FECH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferrochelatase (FECH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferrochelatase (FECH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferrochelatase (FECH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ferrochelatase, mitochondrial Protein information
  • Uniprot ID HEMH_HUMAN
  • Uniprot AC P22830; A8KA72; Q8IXN1; Q8NAN0;
  • UniGene Hs.365365;
  • GeneID 2235
  • KEGG hsa:2235;
  • Human Ferrochelatase, mitochondrial Protein SEQUENCE
  • SEQUENCE 423 AA; 47862 MW; 3FD50965E8DEABCE CRC64;

    MRSLGANMAA ALRAAGVLLR DPLASSSWRV CQPWRWKSGA AAAAVTTETA

    QHAQGAKPQV QPQKRKPKTG ILMLNMGGPE TLGDVHDFLL RLFLDRDLMT

    LPIQNKLAPF IAKRRTPKIQ EQYRRIGGGS PIKIWTSKQG EGMVKLLDEL

    SPNTAPHKYY IGFRYVHPLT EEAIEEMERD GLERAIAFTQ YPQYSCSTTG

    SSLNAIYRYY NQVGRKPTMK WSTIDRWPTH HLLIQCFADH ILKELDHFPL

    EKRSEVVILF SAHSLPMSVV NRGDPYPQEV SATVQKVMER LEYCNPYRLV

    WQSKVGPMPW LGPQTDESIK GLCERGRKNI LLVPIAFTSD HIETLYELDI

    EYSQVLAKEC GVENIRRAES LNGNPLFSKA LADLVHSHIQ SNELCSKQLT

    LSCPLCVNPV CRETKSFFTS QQL

  • UCSC uc002lgp.5; human. [P22830-1];


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