Mouse Adra1d ELISA Kit

Contact Us
  • Alternative name

    Mouse Alpha-1A adrenergic receptor ELISA Kit;Mouse Alpha-1D adrenoreceptor ELISA Kit;Mouse Alpha-1D adrenoceptor ELISA Kit;Mouse Adra1a ELISA Kit;Mouse Gpcr8 ELISA Kit;

  • Catalog
    E043343
  • species
    Mouse
  • GeneAdra1d
  • Standard CurveMouse Adra1d ELISA Kit
  • Other Species Human ADRA1D ELISA KitPorcine ADRA1D ELISA KitRabbit ADRA1D ELISA KitRat Adra1d ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADRA1D. No significant cross-reactivity or interference between ADRA1D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRA1D and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Adra1d ELISA Kit allows for the in vitro quantitative determination of Adra1d , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses: This ADRA1D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADRA1D. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADRA1D ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADRA1D antibody and an ADRA1D-HRP conjugate. The assay sample and buffer are incubated together with ADRA1D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRA1D concentration since ADRA1D from samples and ADRA1D-HRP conjugate compete for the anti-ADRA1D antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRA1D from the sample, fewer sites are left to bind ADRA1D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRA1D concentration in each sample is interpolated from this standard curve.
  • Mouse Alpha-1D adrenergic receptor Protein information
  • Uniprot ID ADA1D_MOUSE
  • Uniprot AC P97714; Q61619;
  • UniGene Mm.389380;
  • Mouse Alpha-1D adrenergic receptor Protein SEQUENCE
  • SEQUENCE 562 AA; 59858 MW; 6CF15515B5F7FA47 CRC64;

    MTFRDILSVT FEGPRASSST GGSGAGGGAG TVGPEGPAVG GVPGATGGSA

    VVGTGSGEDN QSSTAEAGAA ASGEVNGSAA VGGLVVSAQG VGVGVFLAAF

    ILTAVAGNLL VILSVACNRH LQTVTNYFIV NLAVADLLLS AAVLPFSATM

    EVLGFWPFGR TFCDVWAAVD VLCCTASILS LCTISVDRYV GVRHSLKYPA

    IMTERKAAAI LALLWAVALV VSVGPLLGWK EPVPPDERFC GITEEVGYAI

    FSSVCSFYLP MAVIVVMYCR VYVVARSTTR SLEAGIKREP GKASEVVLRI

    HCRGAATSAK GNPGTQSSKG HTLRSSLSVR LLKFSREKKA AKTLAIVVGV

    FVLCWFPFFF VLPLGSLFPQ LKPSEGVFKV IFWLGYFNSC VNPLIYPCSS

    REFKRAFLRL LRCQCRRRRR RLWPSLRPPL ASLDRRPALR LCPQPAHRTP

    RGSPSPHCTP RPGLRRHAGG AGFGLRPSKA SLRLREWRLL GPLQRPTTQL

    RAKVSSLSHK FRSGGARRAE TACALRSEVE AVSLNVPQDG AEAVICQAYE

    PGDLSNLRET DI





tag

Inquiry About Mouse Adra1d ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!