Human SKP2 ELISA Kit

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  • Alternative name

    Human Cyclin-A/CDK2-associated protein p45 ELISA Kit;Human F-box protein Skp2 ELISA Kit;Human F-box/LRR-repeat protein 1 ELISA Kit;Human p45skp2 ELISA Kit;Human FBXL1 ELISA Kit;Human FBL1 ELISA Kit;Human FLB1 ELISA Kit;Human p45 ELISA Kit;Human S-phase kinase associated protein 2 ELISA Kit;Human S-phase kinase-associated protein 2 ELISA Kit;Human CDK2/cyclin A-associated protein p45 ELISA Kit;Human S-phase kinase-associated protein 2, E3 ubiquitin protein ligase ELISA Kit;

  • Catalog
    E034060
  • species
    Human
  • GeneSKP2
  • Standard CurveHuman SKP2 ELISA Kit
  • Other Species Mouse Skp2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of S-Phase Kinase Associated Protein 2 (SKP2). No significant cross-reactivity or interference between S-Phase Kinase Associated Protein 2 (SKP2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman SKP2 ELISA Kit allows for the in vitro quantitative determination of SKP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 2 (SKP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 2 (SKP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Phase Kinase Associated Protein 2 (SKP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human S-phase kinase-associated protein 2 Protein information
  • Uniprot ID SKP2_HUMAN
  • Uniprot AC Q13309; A8K5E0; B4DJT4; Q8TDZ0; Q8TDZ1; Q9BV69;
  • UniGene Hs.23348;
  • GeneID 6502
  • KEGG hsa:6502;
  • Human S-phase kinase-associated protein 2 Protein SEQUENCE
  • SEQUENCE 424 AA; 47761 MW; F29B7C338A7A37E9 CRC64;

    MHRKHLQEIP DLSSNVATSF TWGWDSSKTS ELLSGMGVSA LEKEEPDSEN

    IPQELLSNLG HPESPPRKRL KSKGSDKDFV IVRRPKLNRE NFPGVSWDSL

    PDELLLGIFS CLCLPELLKV SGVCKRWYRL ASDESLWQTL DLTGKNLHPD

    VTGRLLSQGV IAFRCPRSFM DQPLAEHFSP FRVQHMDLSN SVIEVSTLHG

    ILSQCSKLQN LSLEGLRLSD PIVNTLAKNS NLVRLNLSGC SGFSEFALQT

    LLSSCSRLDE LNLSWCFDFT EKHVQVAVAH VSETITQLNL SGYRKNLQKS

    DLSTLVRRCP NLVHLDLSDS VMLKNDCFQE FFQLNYLQHL SLSRCYDIIP

    ETLLELGEIP TLKTLQVFGI VPDGTLQLLK EALPHLQINC SHFTTIARPT

    IGNKKNQEIW GIKCRLTLQK PSCL

  • UCSC uc003jkc.3; human. [Q13309-1];


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