Mouse Met ELISA Kit

Alternative name
Mouse HGF receptor ELISA Kit;Mouse HGF/SF receptor ELISA Kit;Mouse Proto-oncogene c-Met ELISA Kit;Mouse Scatter factor receptor ELISA Kit;Mouse SF receptor ELISA Kit;Mouse Tyrosine-protein kinase Met ELISA Kit;
Catalog
E046289
species
Mouse
GeneMet
Standard Curve
Other Species Human MET ELISA Kit Human c-MET/HGFR ELISA Kit Human C.met ELISA Kit Human c-MET ELISA Kit Human Met ENK ELISA Kit Mouse C-MET ELISA Kit Mouse C.met ELISA Kit Mouse Met-ENK ELISA Kit Bovine MET ELISA Kit Canine MET ELISA Kit Horse MET ELISA Kit Porcine MET ELISA Kit Rabbit MET ELISA Kit Rat Met ELISA Kit Sheep MET ELISA Kit
SpecificityNatural and recombinant mouse C-MET
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 5pg/ml
Intended UseMouse Met ELISA Kit allows for the in vitro quantitative determination of Met , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for C-MET has been precoated onto 96-well plates. Standards (sf21,E25 - R306 (alpha) &S307 - N929(beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse C-MET amount of sample captured in plate. Background: C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
Mouse Hepatocyte growth factor receptor Protein information
Uniprot ID MET_MOUSE
Uniprot AC P16056; Q62125;
UniGene Mm.86844;
Mouse Hepatocyte growth factor receptor Protein SEQUENCE
SEQUENCE 1379 AA; 153549 MW; FC5CC87FDD8ADED8 CRC64;
MKAPTVLAPG ILVLLLSLVQ RSHGECKEAL VKSEMNVNMK YQLPNFTAET
PIQNVVLHGH HIYLGATNYI YVLNDKDLQK VSEFKTGPVL EHPDCLPCRD
CSSKANSSGG VWKDNINMAL LVDTYYDDQL ISCGSVNRGT CQRHVLPPDN
SADIQSEVHC MFSPEEESGQ CPDCVVSALG AKVLLSEKDR FINFFVGNTI
NSSYPPGYSL HSISVRRLKE TQDGFKFLTD QSYIDVLPEF LDSYPIKYIH
AFESNHFIYF LTVQKETLDA QTFHTRIIRF CSVDSGLHSY MEMPLECILT
EKRRKRSTRE EVFNILQAAY VSKPGANLAK QIGASPSDDI LFGVFAQSKP
DSAEPVNRSA VCAFPIKYVN DFFNKIVNKN NVRCLQHFYG PNHEHCFNRT
LLRNSSGCEA RSDEYRTEFT TALQRVDLFM GRLNQVLLTS ISTFIKGDLT
IANLGTSEGR FMQVVLSRTA HLTPHVNFLL DSHPVSPEVI VEHPSNQNGY
TLVVTGKKIT KIPLNGLGCG HFQSCSQCLS APYFIQCGWC HNQCVRFDEC
PSGTWTQEIC LPAVYKVFPT SAPLEGGTVL TICGWDFGFR KNNKFDLRKT
KVLLGNESCT LTLSESTTNT LKCTVGPAMS EHFNVSVIIS NSRETTQYSA
FSYVDPVITS ISPRYGPQAG GTLLTLTGKY LNSGNSRHIS IGGKTCTLKS
VSDSILECYT PAQTTSDEFP VKLKIDLANR ETSSFSYRED PVVYEIHPTK
SFISGGSTIT GIGKTLNSVS LPKLVIDVHE VGVNYTVACQ HRSNSEIICC
TTPSLKQLGL QLPLKTKAFF LLDGILSKHF DLTYVHNPVF EPFEKPVMIS
MGNENVVEIK GNNIDPEAVK GEVLKVGNQS CESLHWHSGA VLCTVPSDLL
KLNSELNIEW KQAVSSTVLG KVIVQPDQNF AGLIIGAVSI SVVVLLLSGL
FLWMRKRKHK DLGSELVRYD ARVHTPHLDR LVSARSVSPT TEMVSNESVD
YRATFPEDQF PNSSQNGACR QVQYPLTDLS PILTSGDSDI SSPLLQNTVH
IDLSALNPEL VQAVQHVVIG PSSLIVHFNE VIGRGHFGCV YHGTLLDNDG
KKIHCAVKSL NRITDIEEVS QFLTEGIIMK DFSHPNVLSL LGICLRSEGS
PLVVLPYMKH GDLRNFIRNE THNPTVKDLI GFGLQVAKGM KYLASKKFVH
RDLAARNCML DEKFTVKVAD FGLARDMYDK EYYSVHNKTG AKLPVKWMAL
ESLQTQKFTT KSDVWSFGVL LWELMTRGAP PYPDVNTFDI TIYLLQGRRL
LQPEYCPDAL YEVMLKCWHP KAEMRPSFSE LVSRISSIFS TFIGEHYVHV
NATYVNVKCV APYPSLLPSQ DNIDGEGNT

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Category

Mouse Met ELISA Kit

Mouse HGF receptor ELISA Kit;Mouse HGF/SF receptor ELISA Kit;Mouse Proto-oncogene c-Met ELISA Kit;Mouse Scatter factor receptor ELISA Kit;Mouse SF receptor ELISA Kit;Mouse Tyrosine-protein kinase Met ELISA Kit;