Human FADS3 ELISA Kit

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  • Alternative name

    Human cytochrome b5-related protein ELISA Kit;Human CYB5RP ELISA Kit;Human LLCDL3 ELISA Kit;Human fatty acid desaturase 3 ELISA Kit;Human delta-9 fatty acid desaturase ELISA Kit;Human delta-9-desaturase ELISA Kit;Human linoleoyl-CoA desaturase (delta-9-desaturase)-like 3 ELISA Kit;

  • Catalog
    E014544
  • species
    Human
  • GeneFADS3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Fatty Acid Desaturase 3 (FADS3). No significant cross-reactivity or interference between Fatty Acid Desaturase 3 (FADS3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.118ng/mL.
  • Intended UseHuman FADS3 ELISA Kit allows for the in vitro quantitative determination of FADS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Desaturase 3 (FADS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fatty Acid Desaturase 3 (FADS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fatty Acid Desaturase 3 (FADS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fatty Acid Desaturase 3 (FADS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Fatty acid desaturase 3 Protein information
  • Uniprot ID FADS3_HUMAN
  • Uniprot AC Q9Y5Q0; O60426;
  • UniGene Hs.21765;
  • GeneID 3995
  • KEGG hsa:3995;
  • Human Fatty acid desaturase 3 Protein SEQUENCE
  • SEQUENCE 445 AA; 51145 MW; 7840EF6BE055111D CRC64;

    MGGVGEPGPR EGPAQPGAPL PTFCWEQIRA HDQPGDKWLV IERRVYDISR

    WAQRHPGGSR LIGHHGAEDA TDAFRAFHQD LNFVRKFLQP LLIGELAPEE

    PSQDGPLNAQ LVEDFRALHQ AAEDMKLFDA SPTFFAFLLG HILAMEVLAW

    LLIYLLGPGW VPSALAAFIL AISQAQSWCL QHDLGHASIF KKSWWNHVAQ

    KFVMGQLKGF SAHWWNFRHF QHHAKPNIFH KDPDVTVAPV FLLGESSVEY

    GKKKRRYLPY NQQHLYFFLI GPPLLTLVNF EVENLAYMLV CMQWADLLWA

    ASFYARFFLS YLPFYGVPGV LLFFVAVRVL ESHWFVWITQ MNHIPKEIGH

    EKHRDWVSSQ LAATCNVEPS LFTNWFSGHL NFQIEHHLFP RMPRHNYSRV

    APLVKSLCAK HGLSYEVKPF LTALVDIVRS LKKSGDIWLD AYLHQ

  • UCSC uc001nsm.5; human.;



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