Human 17 OHCS ELISA Kit

Contact Us
  • Catalog
  • species
  • Standard CurveHuman 17 OHCS ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EBMZ. No significant cross-reactivity or interference between EBMZ and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EBMZ and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 pmol/ml.
  • Intended UseHuman 17 OHCS ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    Principle of the assay: 17-Hydroxypregnenolone ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-17-Hydroxypregnenolone antibody and an 17-Hydroxypregnenolone-HRP conjugate. The assay sample and buffer are incubated together with 17-Hydroxypregnenolone-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 17-Hydroxypregnenolone concentration since 17-Hydroxypregnenolone from samples and 17-Hydroxypregnenolone-HRP conjugate compete for the anti-17-Hydroxypregnenolone antibody binding site. Since the number of sites is limited, as more sites are occupied by 17-Hydroxypregnenolone from the sample, fewer sites are left to bind 17-Hydroxypregnenolone-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 17-Hydroxypregnenolone concentration in each sample is interpolated from this standard curve.

Inquiry About Human 17 OHCS ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!