Human ATG12 ELISA Kit

Alternative name
Human Autophagy-related protein 12 ELISA Kit;Human APG12-like ELISA Kit;Human APG12 ELISA Kit;Human APG12L ELISA Kit;Human FBR93 ELISA Kit;Human HAPG12 ELISA Kit;Human autophagy related 12 ELISA Kit;Human ubiquitin-like protein ATG12 ELISA Kit;Human APG12 autophagy 12-like ELISA Kit;Human ATG12 autophagy related 12 homolog ELISA Kit;Human Apg12 (autophagy, yeast) homolog ELISA Kit;
Catalog
E003934
species
Human
GeneATG12
SpecificityThis assay has high sensitivity and excellent specificity for detection of human ATG12. No significant cross-reactivity or interference between human ATG12 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ATG12 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman ATG12 ELISA Kit allows for the in vitro quantitative determination of ATG12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ubiquitin-like protein ATG12 Protein information
Uniprot ID ATG12_HUMAN
Uniprot AC O94817; Q6PJV2;
UniGene Hs.264482;
GeneID 9140
KEGG hsa:9140;
Human Ubiquitin-like protein ATG12 Protein SEQUENCE
SEQUENCE 140 AA; 15113 MW; 109258521C2D194F CRC64;
MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD
TKKKIDILLK AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL
FIYVNQSFAP SPDQEVGTLY ECFGSDGKLV LHYCKSQAWG
UCSC uc003krh.4; human. [O94817-1];

Human ATG12 ELISA Kit

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