-
Alternative name
Lipid phosphate phosphatase delta ELISA KIT; Phosphatidic acid phosphatase delta ELISA KIT; Sphingoid phosphate phosphatase 1 ELISA KIT; AtSSP1 ELISA KIT; Sphingosine-1-phosphate phosphatase ELISA KIT; AtSPPASELPPD ELISA KIT; SPP1 ELISA KIT; AtLPPD ELISA KIT; AtSSP1 ELISA KIT; AtSPPASE ELISA KIT
-
Catalog
E036435
-
species
Human
-
GeneAT3G58490
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity< 0.094ng/ml
-
Intended UseHuman AT3G58490 ELISA Kit allows for the in vitro quantitative determination of AT3G58490 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Sphingosine 1 Phosphate. During the reaction, Sphingosine 1 Phosphate in the sample or standard competes with a fixed amount of Sphingosine 1 Phosphate on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Sphingosine 1 Phosphate. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Sphingosine 1 Phosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-