SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed.
Intended UseHuman AT3G58490 ELISA Kit allows for the in vitro quantitative determination of AT3G58490 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Sphingosine 1 Phosphate. During the reaction, Sphingosine 1 Phosphate in the sample or standard competes with a fixed amount of Sphingosine 1 Phosphate on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Sphingosine 1 Phosphate. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Sphingosine 1 Phosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.