Human RPL8 ELISA Kit

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  • Alternative name

    Human L8 ELISA Kit;Human ribosomal protein L8 ELISA Kit;Human 60S ribosomal protein L8 ELISA Kit;

  • Catalog
    E042195
  • species
    Human
  • GeneRPL8
  • Standard CurveHuman RPL8 ELISA Kit
  • Other Species Mouse Rpl8 ELISA KitBovine RPL8 ELISA KitRat Rpl8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RPL8. No significant cross-reactivity or interference between RPL8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPL8 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RPL8 ELISA Kit allows for the in vitro quantitative determination of RPL8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This RPL8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPL8. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RPL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPL8 antibody and an RPL8-HRP conjugate. The assay sample and buffer are incubated together with RPL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPL8 concentration since RPL8 from samples and RPL8-HRP conjugate compete for the anti-RPL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPL8 from the sample, fewer sites are left to bind RPL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL8 concentration in each sample is interpolated from this standard curve.
  • Human 60S ribosomal protein L8 Protein information
  • Uniprot ID RL8_HUMAN
  • Uniprot AC P62917; A8K094; D3DWN2; P25120; Q567Q7; Q969V7; Q9BWQ9;
  • UniGene Hs.178551;
  • GeneID 6132
  • KEGG hsa:6132;
  • Human 60S ribosomal protein L8 Protein SEQUENCE
  • SEQUENCE 257 AA; 28025 MW; CE1610449749318B CRC64;

    MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH

    DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN

    VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL

    PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR

    GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK

    TVQEKEN

  • UCSC uc003zeb.4; human.;


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