Mouse Grk5 ELISA Kit

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  • Alternative name

    Mouse g protein-coupled receptor kinase GRK5 ELISA Kit;Mouse Gprk5 ELISA Kit;Mouse G protein-coupled receptor kinase 5 ELISA Kit;

  • Catalog
    E053587
  • species
    Mouse
  • GeneGrk5
  • Standard CurveMouse Grk5 ELISA Kit
  • Other Species Human GRK5 ELISA KitBovine GRK5 ELISA KitRat Grk5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GRK5. No significant cross-reactivity or interference between GRK5 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.115ng/mL.
  • Intended UseMouse Grk5 ELISA Kit allows for the in vitro quantitative determination of Grk5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GRK5 in mouse tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRK5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GRK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GRK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse G protein-coupled receptor kinase 5 Protein information
  • Uniprot ID GRK5_MOUSE
  • Uniprot AC Q8VEB1; O70292; O70297;
  • UniGene Mm.279400; Mm.482751;
  • GeneID 14773
  • KEGG mmu:14773;
  • Mouse G protein-coupled receptor kinase 5 Protein SEQUENCE
  • SEQUENCE 590 AA; 67732 MW; F47D87397B1A2399 CRC64;

    MELENIVANT VLLKAREGGG GKRKGKSKKW KEILKFPHIS QCEDLRRTID

    RDYYSLCDKQ PIGRLLFRQF CETRPGLECY IQFLDLVAEY EITPDENLGA

    KGKEIMTKYL TPKSPVFIAQ VGQDLVSQTE KKLLQSPCKE LFSACAQSVH

    DYLKGDPFHE YLDSMYFDRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV

    CACQVRATGK MYACKRLEKK RIKKRKGESM ALNEKQILEK VNSQFVVNLA

    YAYETKDALC LVLTIMNGGD LKFHIYNMGN PGFEEERALF YAAEILCGLE

    DLHRENTVYR DLKPENILLD DYGHIRISDL GLAVKIPEGD LIRGRVGTVG

    YMAPEVLNNQ RYGLSPDYWG LGCLIYEMIE GQSPFRGRKE KVKREEVDRR

    VLETEEVYSS KFSEEAKSIC NMLLTKDSKQ RLGCQEEGAA EVKRHPFFRN

    MNFKRLEAGM LDPPFVPDPR AVYCKDVLDI EQFSTVKGVN LDHTDDDFYS

    KFSTGSVPIP WQNEMIETEC FKELNVFGPN GTLSPDLNRS QPPEPPKKGL

    FHRLFRRQHQ SNSKSSPTPK TSCNHRINSN HINSNSTGSS

  • UCSC uc008ice.2; mouse.;


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