Human MAPK12 ELISA Kit

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  • Alternative name

    Human MAP kinase 12 ELISA Kit;Human MAPK 12 ELISA Kit;Human extracellular signal-regulated kinase 6 ELISA Kit;Human ERK-6 ELISA Kit;Human mitogen-activated protein kinase p38 gamma ELISA Kit;Human MAP kinase p38 gamma ELISA Kit;Human stress-activated protein kinase 3 ELISA Kit;Human ERK6 ELISA Kit;Human SAPK3 ELISA Kit;Human ERK3 ELISA Kit;Human P38GAMMA ELISA Kit;Human PRKM12 ELISA Kit;Human SAPK-3 ELISA Kit;Human mitogen-activated protein kinase 12 ELISA Kit;Human mitogen-activated protein kinase 3 ELISA Kit;

  • Catalog
    E024699
  • species
    Human
  • GeneMAPK12
  • Standard CurveHuman MAPK12 ELISA Kit
  • Other Species Mouse Mapk12 ELISA KitRat Mapk12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAPK12. No significant cross-reactivity or interference between MAPK12 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human MAPK12 is typically less than 0.072ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by ad
  • Intended UseHuman MAPK12 ELISA Kit allows for the in vitro quantitative determination of MAPK12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPK12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MAPK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPK12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MAPK12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mitogen-activated protein kinase 12 Protein information
  • Uniprot ID MK12_HUMAN
  • Uniprot AC P53778; Q14260; Q6IC53; Q99588; Q99672;
  • UniGene Hs.432642;
  • GeneID 6300
  • KEGG hsa:6300;
  • Human Mitogen-activated protein kinase 12 Protein SEQUENCE
  • SEQUENCE 367 AA; 41940 MW; EF680401D8E40610 CRC64;

    MSSPPPARSG FYRQEVTKTA WEVRAVYRDL QPVGSGAYGA VCSAVDGRTG

    AKVAIKKLYR PFQSELFAKR AYRELRLLKH MRHENVIGLL DVFTPDETLD

    DFTDFYLVMP FMGTDLGKLM KHEKLGEDRI QFLVYQMLKG LRYIHAAGII

    HRDLKPGNLA VNEDCELKIL DFGLARQADS EMTGYVVTRW YRAPEVILNW

    MRYTQTVDIW SVGCIMAEMI TGKTLFKGSD HLDQLKEIMK VTGTPPAEFV

    QRLQSDEAKN YMKGLPELEK KDFASILTNA SPLAVNLLEK MLVLDAEQRV

    TAGEALAHPY FESLHDTEDE PQVQKYDDSF DDVDRTLDEW KRVTYKEVLS

    FKPPRQLGAR VSKETPL

  • UCSC uc003bkl.2; human. [P53778-1];


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