Human ARG ELISA Kit

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  • Catalog
    E003599
  • species
    Human
  • GeneARG
  • Standard CurveHuman ARG ELISA Kit
  • Other Species Human ARG ELISA KitHuman L-Arg ELISA KitMouse ARG ELISA KitMouse L-Arg ELISA KitBovine ARG ELISA KitCanine ARG ELISA KitChicken ARG ELISA KitGeneral ARG ELISA KitPorcine ARG ELISA KitRabbit ARG ELISA KitRat ARG ELISA KitCamel ARG ELISA KitGoat ARG ELISA KitGuinea Pig ARG ELISA KitHamster ARG ELISA KitHorse ARG ELISA KitMonkey ARG ELISA KitSheep ARG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Arginase (ARG). No significant cross-reactivity or interference between Arginase (ARG) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.29ng/mL.
  • Intended UseHuman ARG ELISA Kit allows for the in vitro quantitative determination of ARG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginase (ARG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginase (ARG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginase (ARG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginase (ARG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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