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Catalog
E035685
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species
Human
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GenesELAF
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human sELAF. No significant cross-reactivity or interference between human sELAF and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.78 ng/ml.
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Intended UseHuman sELAF ELISA Kit allows for the in vitro quantitative determination of sELAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sELAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sELAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sELAF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sELAF bound in the initial step. The color development is stopped and the intensity of the color is measured.
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