Human PYD ELISA Kit

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  • Catalog
    E032527
  • species
    Human
  • GenePYD
  • Standard CurveHuman PYD ELISA Kit
  • Other Species Human OH PYD ELISA KitHuman OH-PYD ELISA KitHuman PYD ELISA KitMouse OH-PYD ELISA KitMouse PYD ELISA KitBovine PYD ELISA KitCanine PYD ELISA KitChicken PYD ELISA KitGeneral PYD ELISA KitPorcine PYD ELISA KitRabbit PYD ELISA KitRat PYD ELISA KitCamel PYD ELISA KitGoat PYD ELISA KitGuinea Pig PYD ELISA KitHamster PYD ELISA KitHorse PYD ELISA KitMonkey PYD ELISA KitSheep PYD ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of C-PYD. No significant cross-reactivity or interference between C-PYD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between C-PYD and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PYD ELISA Kit allows for the in vitro quantitative determination of PYD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: C-PYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-C-PYD antibody and an C-PYD-HRP conjugate. The assay sample and buffer are incubated together with C-PYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C-PYD concentration since C-PYD from samples and C-PYD-HRP conjugate compete for the anti-C-PYD antibody binding site. Since the number of sites is limited, as more sites are occupied by C-PYD from the sample, fewer sites are left to bind C-PYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C-PYD concentration in each sample is interpolated from this standard curve.



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