-
Catalog
E029919
-
species
Human
-
SpecificityThe PMN Elastase test is specific human PMN elastase only, respectively the PMN elastase/alpha1-PI complex.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityThe lower detection limit for PMN Elastase was 0.2 ng/ml.
-
Intended UseHuman PRAM1 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Categories/FamilyImmunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
-
Product Description
specificalPrinciple of the assay: The test kit is a solid phase enzyme immunometric assay (ELISA) in the microplate format, designed for the quantitative measurement of the complex of human PMN elastase and alpha1 proteinase inhibitor (alpha1-PI) in plasma. The microplate is coated with a first polyclonal antibody against human PMN elastase (antigen). Calibrators, controls and patient samples are pipetted into the antibody coated microplate. During a 60 minutes incubation present antigens in the sample bind to the antibodies fixed on the inner surface of the wells. Non-reactive sample components are removed by a washing step. Afterwards, a second polyclonal antibody against alpha1-PI, which is labeled with horseradish peroxidase, is added. During a 60 minutes incubation, the PMN elastase/alpha1-PI complex bound to the first antibody is specifically recognized by the enzyme labeled antibodies, and a sandwich complex is formed. An excess of enzyme conjugate is washed out. A chromogenic substrate, TMB (3,3',5,5'-Tetra-Methyl-Benzidine), is added. During a 20 minutes incubation, the substrate is converted to a colored endproduct (blue) by the fixed enzyme. Enzyme reaction is stopped by dispensing of hydrochloric acid as stop solution (change from blue to yellow). The color intensitiy is direct proportional to the concentration of PMN elastase present in the sample. The optical density of the color solution is measured with a microplate reader at 450 nm. Bi-chromatic measurement with a 600 - 690 nm reference filter is recommended.
-