Human PPIase A ELISA Kit;Human Cyclophilin A ELISA Kit;Human cyclosporin A-binding protein ELISA Kit;Human rotamase A ELISA Kit;Human CYPA ELISA Kit;Human CYPH ELISA Kit;Human HEL-S-69p ELISA Kit;Human peptidylprolyl isomerase A ELISA Kit;Human peptidyl-prolyl cis-trans isomerase A ELISA Kit;Human T cell cyclophilin ELISA Kit;Human epididymis secretory sperm binding protein Li 69p ELISA Kit;Human peptidylprolyl isomerase A (cyclophilin A) ELISA Kit;
Catalog
E028604
species
Human
GenePPIA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PPIA. No significant cross-reactivity or interference between Human PPIA and analogues was observed.
Intended UseHuman PPIA ELISA Kit allows for the in vitro quantitative determination of PPIA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntroduction: Cyclophilins, abbreviated Cyp, are proteins that bind to cyclosporine (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants. These proteins have peptidyl prolyl isomerase activity, which catalyzes the isomerization of peptide bonds from trans form to cis form at proline residues and facilitates protein folding. Cyclophilin A (CyPA) is an abundant intracellular protein that is considered to be the main target of the immunosuppressive drug cyclosporine A. Human Cyclophilin A exists in cytosol, has a beta barrel structure with two alpha helices and a beta-sheet. Other cyclophilins have similar structures to cyclophilin A. The cyclosporin A-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CyPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for CyPA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CyPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CyPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Peptidyl-prolyl cis-trans isomerase A, N-terminally processed Protein information
Uniprot ID
PPIA_HUMAN
Uniprot AC
Q9BTY9; Q9UC61;
UniGene
Hs.356331;
GeneID
5478
KEGG
hsa:5478;
Human Peptidyl-prolyl cis-trans isomerase A, N-terminally processed Protein SEQUENCE