Human PON3 ELISA Kit

Alternative name
Human paraoxonase 3 ELISA Kit;Human serum paraoxonase/lactonase 3 ELISA Kit;Human arylesterase 3 ELISA Kit;Human paraoxanase-3 ELISA Kit;
Catalog
E028304
species
Human
GenePON3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Paraoxonase 3 (PON3). No significant cross-reactivity or interference between Paraoxonase 3 (PON3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 37pg/mL.
Intended UseHuman PON3 ELISA Kit allows for the in vitro quantitative determination of PON3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 3 (PON3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 3 (PON3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 3 (PON3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paraoxonase 3 (PON3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serum paraoxonase/lactonase 3 Protein information
Uniprot ID PON3_HUMAN
Uniprot AC Q15166; A4D1H8; O75855; O76060; Q6IRU9; Q8IX97; Q9BZH9;
UniGene Hs.440967;
GeneID 5446
KEGG hsa:5446;
Human Serum paraoxonase/lactonase 3 Protein SEQUENCE
SEQUENCE 354 AA; 39607 MW; 1B861B35E8533921 CRC64;
MGKLVALVLL GVGLSLVGEM FLAFRERVNA SREVEPVEPE NCHLIEELES
GSEDIDILPS GLAFISSGLK YPGMPNFAPD EPGKIFLMDL NEQNPRAQAL
EISGGFDKEL FNPHGISIFI DKDNTVYLYV VNHPHMKSTV EIFKFEEQQR
SLVYLKTIKH ELLKSVNDIV VLGPEQFYAT RDHYFTNSLL SFFEMILDLR
WTYVLFYSPR EVKVVAKGFC SANGITVSAD QKYVYVADVA AKNIHIMEKH
DNWDLTQLKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LNYNPEDPPG
SEVLRIQNVL SEKPRVSTVY ANNGSVLQGT SVASVYHGKI LIGTVFHKTL
YCEL
UCSC uc003unt.4; human.;

Category

Human PON3 ELISA Kit

Human paraoxonase 3 ELISA Kit;Human serum paraoxonase/lactonase 3 ELISA Kit;Human arylesterase 3 ELISA Kit;Human paraoxanase-3 ELISA Kit;