Human FLOT2 ELISA Kit

Alternative name
Human epidermal surface antigen ELISA Kit;Human ESA ELISA Kit;Human membrane component chromosome 17 surface marker 1 ELISA Kit;Human ESA1 ELISA Kit;Human M17S1 ELISA Kit;Human ECS-1 ELISA Kit;Human ECS1 ELISA Kit;Human flotillin 2 ELISA Kit;Human flotillin-2 ELISA Kit;Human Flotillin 2 (epidermal surface antigen 1) ELISA Kit;Human membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) ELISA Kit;
Catalog
E015237
species
Human
GeneFLOT2
Standard Curve
Other Species Mouse Flot2 ELISA Kit Bovine FLOT2 ELISA Kit Rat Flot2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Flotillin 2 (FLOT2). No significant cross-reactivity or interference between Flotillin 2 (FLOT2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.273ng/mL.
Intended UseHuman FLOT2 ELISA Kit allows for the in vitro quantitative determination of FLOT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin 2 (FLOT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flotillin 2 (FLOT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flotillin 2 (FLOT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flotillin 2 (FLOT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Flotillin-2 Protein information
Uniprot ID FLOT2_HUMAN
Uniprot AC Q14254;
UniGene Hs.514038;
GeneID 2319
KEGG hsa:2319;
Human Flotillin-2 Protein SEQUENCE
SEQUENCE 428 AA; 47064 MW; F690BFE0151D3BD7 CRC64;
MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM
TLQPRCEDVE TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK
NVVLQTLEGH LRSILGTLTV EQIYQDRDQF AKLVREVAAP DVGRMGIEIL
SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD IGVAEAERDA GIREAECKKE
MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA YELQGAREQQ
KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI
AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK
YGDAAKMALV LEALPQIAAK IAAPLTKVDE IVVLSGDNSK VTSEVNRLLA
ELPASVHALT GVDLSKIPLI KKATGVQV
UCSC uc002hdc.4; human.;

Related products with Human FLOT2 ELISA Kit

Category

Human FLOT2 ELISA Kit