Human FLOT2 ELISA Kit

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  • Alternative name

    Human epidermal surface antigen ELISA Kit;Human ESA ELISA Kit;Human membrane component chromosome 17 surface marker 1 ELISA Kit;Human ESA1 ELISA Kit;Human M17S1 ELISA Kit;Human ECS-1 ELISA Kit;Human ECS1 ELISA Kit;Human flotillin 2 ELISA Kit;Human flotillin-2 ELISA Kit;Human Flotillin 2 (epidermal surface antigen 1) ELISA Kit;Human membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) ELISA Kit;

  • Catalog
    E015237
  • species
    Human
  • GeneFLOT2
  • Standard CurveHuman FLOT2 ELISA Kit
  • Other Species Mouse Flot2 ELISA KitBovine FLOT2 ELISA KitRat Flot2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Flotillin 2 (FLOT2). No significant cross-reactivity or interference between Flotillin 2 (FLOT2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.273ng/mL.
  • Intended UseHuman FLOT2 ELISA Kit allows for the in vitro quantitative determination of FLOT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Flotillin 2 (FLOT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Flotillin 2 (FLOT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Flotillin 2 (FLOT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Flotillin 2 (FLOT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Flotillin-2 Protein information
  • Uniprot ID FLOT2_HUMAN
  • Uniprot AC Q14254;
  • UniGene Hs.514038;
  • GeneID 2319
  • KEGG hsa:2319;
  • Human Flotillin-2 Protein SEQUENCE
  • SEQUENCE 428 AA; 47064 MW; F690BFE0151D3BD7 CRC64;

    MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM

    TLQPRCEDVE TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK

    NVVLQTLEGH LRSILGTLTV EQIYQDRDQF AKLVREVAAP DVGRMGIEIL

    SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD IGVAEAERDA GIREAECKKE

    MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA YELQGAREQQ

    KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI

    AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK

    YGDAAKMALV LEALPQIAAK IAAPLTKVDE IVVLSGDNSK VTSEVNRLLA

    ELPASVHALT GVDLSKIPLI KKATGVQV

  • UCSC uc002hdc.4; human.;


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