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Human HNF1B ELISA Kit

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  • Alternative name

    Human HNF-1-beta ELISA Kit;Human HNF-1B ELISA Kit;Human homeoprotein LFB3 ELISA Kit;Human Transcription factor 2 ELISA Kit;Human TCF-2 ELISA Kit;Human Variant hepatic nuclear factor 1 ELISA Kit;Human VHNF1 ELISA Kit;Human TCF2 ELISA Kit;Human FJHN ELISA Kit;Human HNF1beta ELISA Kit;Human HNF2 ELISA Kit;Human HPC11 ELISA Kit;Human LF-B3 ELISA Kit;Human LFB3 ELISA Kit;Human MODY5 ELISA Kit;Human HNF1 homeobox B ELISA Kit;Human hepatocyte nuclear factor 1-beta ELISA Kit;Human HNF1 beta A ELISA Kit;Human transcription factor 2, hepatic ELISA Kit;
  • Catalog
    E018400
  • species
    Human
  • GeneHNF1B
  • Standard CurveHuman HNF1B ELISA Kit
  • Other Species Mouse Hnf1b ELISA KitPorcine HNF1B ELISA KitRat Hnf1b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Beta (HNF1b). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Beta (HNF1b) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.047ng/mL.
  • Intended UseHuman HNF1B ELISA Kit allows for the in vitro quantitative determination of HNF1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta (HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta (HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta (HNF1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hepatocyte nuclear factor 1-beta Protein information
  • Uniprot ID HNF1B_HUMAN
  • Uniprot AC P35680; B4DKM3; E0YMJ9;
  • UniGene Hs.191144;
  • GeneID 6928
  • KEGG hsa:6928;
  • Human Hepatocyte nuclear factor 1-beta Protein SEQUENCE

  • SEQUENCE 557 AA; 61324 MW; 9E1ECC7423B73980 CRC64;

    MVSKLTSLQQ ELLSALLSSG VTKEVLVQAL EELLPSPNFG VKLETLPLSP

    GSGAEPDTKP VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT

    EEAAEQRAEV DRMLSEDPWR AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL

    SQHLNKGTPM KTQKRAALYT WYVRKQREIL RQFNQTVQSS GNMTDKSSQD

    QLLFLFPEFS QQSHGPGQSD DACSEPTNKK MRRNRFKWGP ASQQILYQAY

    DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF

    ANRRKEEAFR QKLAMDAYSS NQTHSLNPLL SHGSPHHQPS SSPPNKLSGV

    RYSQQGNNEI TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHNLLSPDG

    KMISVSGGGL PPVSTLTNIH SLSHHNPQQS QNLIMTPLSG VMAIAQSLNT

    SQAQSVPVIN SVAGSLAALQ PVQFSQQLHS PHQQPLMQQS PGSHMAQQPF

    MAAVTQLQNS HMYAHKQEPP QYSHTSRFPS AMVVTDTSSI STLTNMSSSK

    QCPLQAW

  • UCSC uc010wdi.3; human. [P35680-1];


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