Human WTIP ELISA Kit

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  • Alternative name

    Human WT1-interacting protein ELISA Kit;Human Wilms tumor 1 interacting protein ELISA Kit;Human Wilms tumor protein 1-interacting protein ELISA Kit;

  • Catalog
    E040596
  • species
    Human
  • GeneWTIP
  • Other Species Mouse Wtip ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.234ng/mL
  • Intended UseHuman WTIP ELISA Kit allows for the in vitro quantitative determination of WTIP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WTIP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WTIP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WTIP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WTIP can be calculated.
  • Human Wilms tumor protein 1-interacting protein Protein information
  • Uniprot ID WTIP_HUMAN
  • Uniprot AC A6NIX2;
  • UniGene Hs.585010;
  • GeneID 126374
  • KEGG hsa:126374;
  • Human Wilms tumor protein 1-interacting protein Protein SEQUENCE
  • SEQUENCE 430 AA; 45124 MW; B95E162B3009B886 CRC64;

    MQRSRAGADE AALLLAGLAL RELEPGCGSP GRGRRGPRPG PGDEAAPALG

    RRGKGSGGPE AGADGLSRGE RGPRRAAVPE LSAQPAGSPR ASLAGSDGGG

    GGGSARSSGI SLGYDQRHGS PRSGRSDPRP GPGPPSVGSA RSSVSSLGSR

    GSAGAYADFL PPGACPAPAR SPEPAGPAPF PLPALPLPPG REGGPSAAER

    RLEALTRELE RALEARTARD YFGICIKCGL GIYGAQQACQ AMGSLYHTDC

    FTCDSCGRRL RGKAFYNVGE KVYCQEDFLY SGFQQTADKC SVCGHLIMEM

    ILQALGKSYH PGCFRCSVCN ECLDGVPFTV DVENNIYCVR DYHTVFAPKC

    ASCARPILPA QGCETTIRVV SMDRDYHVAC YHCEDCGLQL SGEEGRRCYP

    LAGHLLCRRC HLRRLQPGPL PSPTVHVTEL

  • UCSC uc002nvm.4; human.;



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