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Alternative name
Retinol-binding protein 4 ELISA KIT; Plasma retinol-binding protein ELISA KIT; PRBP ELISA KIT; RBPRBP4 ELISA KIT; PRBP ELISA KIT; RBP ELISA KIT
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Catalog
E033401
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species
Human
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GeneRBP
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe detection limit was defined as B0 + 2 SD and determined to be 0.9 mug/l.
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Intended UseHuman RBP ELISA Kit allows for the in vitro quantitative determination of RBP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular; Research Assays; Assay Categories
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Product Description
specificalPrinciple of the assay: This ELISA can be used for determination of retinol-binding protein (RBP)/RBP4 in plasma, serum and urine. In a first incubation step, RBP/RBP4 in the samples is bound to polyclonal rabbit anti RBP/RBP4 antibodies, immobilized on the microtitre plate. A peroxidase-conjugated anti RBP/RBP4 antibody is used for detection and determination, and tetramethylbenzidine (TMB) as a peroxidase substrate. A dose response curve of absorbance unit (optical density at 450 nm) vs. concentration is generated using the values obtained from standard. RBP/RBP4 present in the samples is determined directly from this curve.
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