Human CHRM3 ELISA Kit

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  • Alternative name

    Human EGBRS ELISA Kit;Human HM3 ELISA Kit;Human PBS ELISA Kit;Human cholinergic receptor muscarinic 3 ELISA Kit;Human muscarinic acetylcholine receptor M3 ELISA Kit;Human acetylcholine receptor, muscarinic 3 ELISA Kit;Human m3 muscarinic receptor ELISA Kit;

  • Catalog
    E008270
  • species
    Human
  • GeneCHRM3
  • Standard CurveHuman CHRM3 ELISA Kit
  • Other Species Mouse Chrm3 ELISA KitBovine CHRM3 ELISA KitChicken CHRM3 ELISA KitPorcine CHRM3 ELISA KitRat Chrm3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Muscarinic 3 (CHRM3). No significant cross-reactivity or interference between Cholinergic Receptor, Muscarinic 3 (CHRM3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.30ng/mL.
  • Intended UseHuman CHRM3 ELISA Kit allows for the in vitro quantitative determination of CHRM3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Muscarinic 3 (CHRM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Muscarinic 3 (CHRM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Muscarinic 3 (CHRM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Muscarinic acetylcholine receptor M3 Protein information
  • Uniprot ID ACM3_HUMAN
  • Uniprot AC P20309; Q0VAJ8; Q4QRI3; Q5VXY2; Q9HB60;
  • UniGene Hs.155736; Hs.7138;
  • GeneID 1131
  • KEGG hsa:1131;
  • Human Muscarinic acetylcholine receptor M3 Protein SEQUENCE
  • SEQUENCE 590 AA; 66128 MW; 5CB473C57B9526E9 CRC64;

    MTLHNNSTTS PLFPNISSSW IHSPSDAGLP PGTVTHFGSY NVSRAAGNFS

    SPDGTTDDPL GGHTVWQVVF IAFLTGILAL VTIIGNILVI VSFKVNKQLK

    TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLAIDYV

    ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFVLW

    APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL

    YWRIYKETEK RTKELAGLQA SGTEAETENF VHPTGSSRSC SSYELQQQSM

    KRSNRRKYGR CHFWFTTKSW KPSSEQMDQD HSSSDSWNNN DAAASLENSA

    SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGMVD

    LERKADKLQA QKSVDDGGSF PKSFSKLPIQ LESAVDTAKT SDVNSSVGKS

    TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLVKEKKAA QTLSAILLAF

    IITWTPYNIM VLVNTFCDSC IPKTFWNLGY WLCYINSTVN PVCYALCNKT

    FRTTFKMLLL CQCDKKKRRK QQYQQRQSVI FHKRAPEQAL

  • UCSC uc001hyp.4; human.;



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