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Human NRAS ELISA Kit

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  • Alternative name

    Human transforming protein N-Ras ELISA Kit;Human HRAS1 ELISA Kit;Human ALPS4 ELISA Kit;Human CMNS ELISA Kit;Human N-ras ELISA Kit;Human NCMS ELISA Kit;Human NRAS1 ELISA Kit;Human NS6 ELISA Kit;Human neuroblastoma RAS viral oncogene homolog ELISA Kit;Human GTPase NRas ELISA Kit;Human N-ras protein part 4 ELISA Kit;Human neuroblastoma RAS viral (v-ras) oncogene homolog ELISA Kit;Human v-ras neuroblastoma RAS viral oncogene homolog ELISA Kit;
  • Catalog
    E026120
  • species
    Human
  • GeneNRAS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NRAS. No significant cross-reactivity or interference between human NRAS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NRAS is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman NRAS ELISA Kit allows for the in vitro quantitative determination of NRAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRAS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRAS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRAS bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human GTPase NRas Protein information
  • Uniprot ID RASN_HUMAN
  • Uniprot AC P01111; Q14971; Q15104; Q15282;
  • UniGene Hs.486502;
  • GeneID 4893
  • KEGG hsa:4893;
  • Human GTPase NRas Protein SEQUENCE

  • SEQUENCE 189 AA; 21229 MW; 6898D3F6815B1EC7 CRC64;

    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET

    CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNSKSF ADINLYREQI

    KRVKDSDDVP MVLVGNKCDL PTRTVDTKQA HELAKSYGIP FIETSAKTRQ

    GVEDAFYTLV REIRQYRMKK LNSSDDGTQG CMGLPCVVM




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