Human PCMT1 ELISA Kit

Alternative name
Human PIMT ELISA Kit;Human L-isoaspartyl protein carboxyl methyltransferase ELISA Kit;Human protein L-isoaspartyl/D-aspartyl methyltransferase ELISA Kit;Human protein-beta-aspartate methyltransferase ELISA Kit;Human protein-L-isoaspartate (D-aspartate) O-methyltransferase ELISA Kit;Human protein-L-isoaspartate(D-aspartate) O-methyltransferase ELISA Kit;
Catalog
E031668
species
Human
GenePCMT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein L-Isoaspartate-O-Methyltransferase (PCMT1). No significant cross-reactivity or interference between Protein L-Isoaspartate-O-Methyltransferase (PCMT1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 13.4pg/mL.
Intended UseHuman PCMT1 ELISA Kit allows for the in vitro quantitative determination of PCMT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein L-Isoaspartate-O-Methyltransferase (PCMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein L-Isoaspartate-O-Methyltransferase (PCMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein L-Isoaspartate-O-Methyltransferase (PCMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein-L-isoaspartate(D-aspartate) O-methyltransferase Protein information
Uniprot ID PIMT_HUMAN
Uniprot AC Q96II9; Q99625; Q9BQV7; Q9BQV8; Q9NP03;
UniGene Hs.279257;
GeneID 5110
KEGG hsa:5110;
Human Protein-L-isoaspartate(D-aspartate) O-methyltransferase Protein SEQUENCE
SEQUENCE 227 AA; 24636 MW; 4EB1122379C8040A CRC64;
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKCNPYMDSP
QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV
GCTGKVIGID HIKELVDDSV NNVRKDDPTL LSSGRVQLVV GDGRMGYAEE
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
GSIKMKPLMG VIYVPLTDKE KQWSRWK
UCSC uc011eeg.3; human. [P22061-1];

Human PCMT1 ELISA Kit

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