Mouse Pdk2 ELISA Kit

Alternative name
Mouse Pyruvate dehydrogenase kinase isoform 2 ELISA Kit;Mouse PDH kinase 2 ELISA Kit;Mouse pyruvate dehydrogenase kinase, isoenzyme 2 ELISA Kit;Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial ELISA Kit;Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial ELISA Kit;Mouse pyruvate dehydrogenase 2 ELISA Kit;Mouse pyruvate dehydrogenase kinase, isozyme 2 ELISA Kit;
Catalog
E065672
species
Mouse
GenePdk2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseMouse Pdk2 ELISA Kit allows for the in vitro quantitative determination of Pdk2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial Protein information
Uniprot ID PDK2_MOUSE
Uniprot AC Q9JK42; Q8VC63;
UniGene Mm.29768;
GeneID 18604
KEGG mmu:18604;
Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial Protein SEQUENCE
SEQUENCE 407 AA; 46041 MW; DBB9F3F967C6A5B1 CRC64;
MRWVRALLKN ASLAGAPKYI EHFSKFSPSP LSMKQFLDFG SSNACEKTSF
TFLRQELPVR LANIMKEINL LPDRVLGTPS VQLVQSWYVQ SLLDIMEFLD
KDPEDHRTLS QFTDALVTIR NRHNDVVPTM AQGVLEYKDT YGDDPVSNQN
IQYFLDRFYL SRISIRMLIN QHTLIFDGST NPAHPKHIGS IDPNCSVSDV
VKDAYDMAKL LCDKYYMASP DLEIQEVNAT NANQPIHMVY VPSHLYHMLF
ELFKNAMRAT VESHESSLTL PPIKIMVALG EEDLSIKMSD RGGGVPLRKI
ERLFSYMYST APTPQPGTGG TPLAGFGYGL PISRLYAKYF QGDLQLFSME
GFGTDAVIYL KALSTDSVER LPVYNKSAWR HYQTIQEAGD WCVPSTEPKN
TSTYRVS
UCSC uc007kzu.2; mouse.;

Category

Mouse Pdk2 ELISA Kit