Human IFNA4 ELISA Kit

Alternative name
Human IFN-alpha-4 ELISA Kit;Human interferon alpha-4B ELISA Kit;Human interferon alpha-76 ELISA Kit;Human interferon alpha-M1 ELISA Kit;Human IFN-alpha4a ELISA Kit;Human INFA4 ELISA Kit;Human interferon alpha 4 ELISA Kit;Human interferon alpha-4 ELISA Kit;
Catalog
E020248
species
Human
GeneIFNA4
SpecificityThis assay has high sensitivity and excellent specificity for detection of IFNa4. No significant cross-reactivity or interference between IFNa4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of IFNa4 is typically less than 5.4pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman IFNA4 ELISA Kit allows for the in vitro quantitative determination of IFNA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IFNa4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IFNa4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IFNa4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IFNa4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Interferon alpha-4 Protein information
Uniprot ID IFNA4_HUMAN
Uniprot AC VIQEVGVE ETPLMNEDSI LAVRKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSLSFSTN
UniGene Hs.1510;
GeneID 3441
KEGG hsa:3441;
Human Interferon alpha-4 Protein SEQUENCE
SEQUENCE 189 AA; 21808 MW; 828DF9C33ABC337F CRC64;
MALSFSLLMA VLVLSYKSIC SLGCDLPQTH SLGNRRALIL LAQMGRISHF
SCLKDRHDFG FPEEEFDGHQ FQKAQAISVL HEMIQQTFNL FSTEDSSAAW
EQSLLEKFST ELYQQLNDLE ACVIQEVGVE ETPLMNEDSI LAVRKYFQRI
TLYLTEKKYS PCAWEVVRAE IMRSLSFSTN LQKRLRRKD
UCSC uc003zon.3; human.;

Category

Human IFNA4 ELISA Kit

Human IFN-alpha-4 ELISA Kit;Human interferon alpha-4B ELISA Kit;Human interferon alpha-76 ELISA Kit;Human interferon alpha-M1 ELISA Kit;Human IFN-alpha4a ELISA Kit;Human INFA4 ELISA Kit;Human interferon alpha 4 ELISA Kit;Human interferon alpha-4 ELISA Kit;