Human LOX 1 ELISA Kit

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  • Alternative name

    Oxidized low-density lipoprotein receptor 1 ELISA KIT; Lectin-like oxidized LDL receptor 1 ELISA KIT; LOX-1 ELISA KIT; Lectin-like oxLDL receptor 1 ELISA KIT; Lectin-type oxidized LDL receptor 1Cleaved into the following chain:Oxidized low-density lipoprotein receptor 1, soluble formOlr1 ELISA KIT; Lox1 ELISA KIT; Oldlr1 ELISA KIT; Ox-LDL receptor 1 ELISA KIT; LOX-1 ELISA KIT; Lectin-like oxLDL receptor 1 ELISA KIT

  • Catalog
  • species
  • GeneLOX 1
  • Standard CurveHuman LOX 1 ELISA Kit
  • Other Species Human LOX-1 ELISA KitMouse LOX-1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman LOX 1 ELISA Kit allows for the in vitro quantitative determination of LOX 1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: LOX-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LOX-1 antibody and an LOX-1-HRP conjugate. The assay sample and buffer are incubated together with LOX-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX-1 concentration since LOX-1 from samples and LOX-1-HRP conjugate compete for the anti-LOX-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX-1 from the sample, fewer sites are left to bind LOX-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX-1 concentration in each sample is interpolated from this standard curve.

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