Human GDI1 ELISA Kit

Alternative name
Human rab GDI alpha ELISA Kit;Human guanosine diphosphate dissociation inhibitor 1 ELISA Kit;Human GDI-1 ELISA Kit;Human oligophrenin-2 ELISA Kit;Human protein XAP-4 ELISA Kit;Human GDIL ELISA Kit;Human OPHN2 ELISA Kit;Human RABGDIA ELISA Kit;Human XAP4 ELISA Kit;Human 1A ELISA Kit;Human MRX41 ELISA Kit;Human MRX48 ELISA Kit;Human RABGD1A ELISA Kit;Human XAP-4 ELISA Kit;Human GDP dissociation inhibitor 1 ELISA Kit;Human rab GDP dissociation inhibitor alpha ELISA Kit;Human mental retardation, X-linked 48 ELISA Kit;Human rab GDP-dissociation inhibitor, alpha ELISA Kit;Human testis secretory sperm-binding protein Li 208a ELISA Kit;
Catalog
E016313
species
Human
GeneGDI1
Standard Curve
Other Species Mouse Gdi1 ELISA Kit Bovine GDI1 ELISA Kit Canine GDI1 ELISA Kit Monkey GDI1 ELISA Kit Rat Gdi1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman GDI1 ELISA Kit allows for the in vitro quantitative determination of GDI1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: GDI-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDI-1. Standards or samples are then added to the microtiter plate wells and GDI-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDI-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for GDI-1 are added to each well to "sandwich" the GDI-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDI-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDI-1 concentration in each sample is interpolated from this standard curve.
Human Rab GDP dissociation inhibitor alpha Protein information
Uniprot ID GDIA_HUMAN
Uniprot AC P31150; P50394; Q6FG50; Q7Z2G6; Q7Z2G9; Q7Z2H5; Q7Z2I6;
UniGene Hs.595950; Hs.74576;
GeneID 2664
KEGG hsa:2664;
Human Rab GDP dissociation inhibitor alpha Protein SEQUENCE
SEQUENCE 447 AA; 50583 MW; BC283A445E50A652 CRC64;
MDEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESSSITPLE
ELYKRFQLLE GPPESMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL
DFKVVEGSFV YKGGKIYKVP STETEALASN LMGMFEKRRF RKFLVFVANF
DENDPKTFEG VDPQTTSMRD VYRKFDLGQD VIDFTGHALA LYRTDDYLDQ
PCLETVNRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM
LNKPVDDIIM ENGKVVGVKS EGEVARCKQL ICDPSYIPDR VRKAGQVIRI
ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISYA HNVAAQGKYI
AIASTTVETT DPEKEVEPAL ELLEPIDQKF VAISDLYEPI DDGCESQVFC
SCSYDATTHF ETTCNDIKDI YKRMAGTAFD FENMKRKQND VFGEAEQ
UCSC uc004fli.5; human.;

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Human GDI1 ELISA Kit