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Alternative name
Acetylcholine ELISA Kit,ACH ELISA Kit
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Catalog
E000252
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species
Human
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GeneACH
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Other Species
Human ACh ELISA KitMouse ACH ELISA KitBovine ACH ELISA KitCanine ACH ELISA KitChicken ACH ELISA KitGeneral ACH ELISA KitPorcine ACH ELISA KitRabbit ACH ELISA KitRat ACH ELISA KitCamel ACH ELISA KitGoat ACH ELISA KitGuinea Pig ACH ELISA KitHamster ACH ELISA KitHorse ACH ELISA KitMonkey ACH ELISA KitSheep ACH ELISA Kit
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity15pg/ml
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Intended UseHuman ACH ELISA Kit allows for the in vitro quantitative determination of ACH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHuman ACH ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to ACH, During the reaction,ACH in the sample or standard competes with a fixed amount of biotin-labeled ACH for sites on a pre-coated Monoclonal antibody specific to ACH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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