Human MDK ELISA Kit

Alternative name
Human MK ELISA Kit;Human amphiregulin-associated protein ELISA Kit;Human ARAP ELISA Kit;Human midgestation and kidney protein ELISA Kit;Human neurite outgrowth-promoting factor 2 ELISA Kit;Human Neurite outgrowth-promoting protein ELISA Kit;Human MK1 ELISA Kit;Human NEGF2 ELISA Kit;Human midkine (neurite growth-promoting factor 2) ELISA Kit;Human midkine ELISA Kit;Human retinoic acid inducible factor ELISA Kit;
Catalog
E024530
species
Human
GeneMDK
SpecificityNatural and recombinant human Midkine
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman MDK ELISA Kit allows for the in vitro quantitative determination of MDK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Midkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Midkine has been precoated onto 96-well plates. Standards(E.coli, K23-D143) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Midkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Midkine amount of sample captured in plate. Background: Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPzeta), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase (ALK) and syndecans is considered to be its receptor.
Human Midkine Protein information
Uniprot ID MK_HUMAN
Uniprot AC P21741; Q2LEK4; Q9UCC7;
UniGene Hs.82045;
GeneID 4192
KEGG hsa:4192;
Human Midkine Protein SEQUENCE
SEQUENCE 143 AA; 15585 MW; 414A627FF39953C3 CRC64;
MQHRGFLLLT LLALLALTSA VAKKKDKVKK GGPGSECAEW AWGPCTPSSK
DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT
KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
UCSC uc001nco.5; human. [P21741-1];

Human MDK ELISA Kit

Contact Us