Human MMP 13 ELISA Kit

Contact Us
  • Alternative name

    MMP-13 ELISA KIT; MMP-13

  • Catalog
    E023769
  • species
    Human
  • GeneMMP 13
  • Standard CurveHuman MMP 13 ELISA Kit
  • Other Species Human MMP-13 ELISA KitHuman Pro-MMP-13 ELISA KitMouse MMP-13 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MMP 13 ELISA Kit allows for the in vitro quantitative determination of MMP 13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Principle of the Assay: MMP-13 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MMP-13 antibody and an MMP-13-HRP conjugate. The assay sample and buffer are incubated together with MMP-13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-13 concentration since MMP-13 from samples and MMP-13-HRP conjugate compete for the anti-MMP-13 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-13 from the sample, fewer sites are left to bind MMP-13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-13 concentration in each sample is interpolated from this standard curve.



Inquiry About Human MMP 13 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!