Human ANO5 ELISA Kit

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  • Alternative name

    Human gnathodiaphyseal dysplasia 1 protein ELISA Kit;Human transmembrane protein 16E ELISA Kit;Human GDD1 ELISA Kit;Human TMEM16E ELISA Kit;Human LGMD2L ELISA Kit;Human anoctamin 5 ELISA Kit;Human anoctamin-5 ELISA Kit;Human integral membrane protein GDD1 ELISA Kit;

  • Catalog
    E002460
  • species
    Human
  • GeneANO5
  • Other Species Mouse Ano5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ANO5. No significant cross-reactivity or interference between ANO5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ANO5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ANO5 ELISA Kit allows for the in vitro quantitative determination of ANO5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This ANO5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ANO5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ANO5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ANO5 antibody and an ANO5-HRP conjugate. The assay sample and buffer are incubated together with ANO5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANO5 concentration since ANO5 from samples and ANO5-HRP conjugate compete for the anti-ANO5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANO5 from the sample, fewer sites are left to bind ANO5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANO5 concentration in each sample is interpolated from this standard curve.
  • Human Anoctamin-5 Protein information
  • Uniprot ID ANO5_HUMAN
  • Uniprot AC Q75V66;
  • UniGene Hs.154329;
  • GeneID 203859
  • KEGG hsa:203859;
  • Human Anoctamin-5 Protein SEQUENCE
  • SEQUENCE 913 AA; 107188 MW; 98BC40318678C073 CRC64;

    MGDPDLLEVL AEEGEKVNKH IDYSFQMSEQ SLSSRETSFL INEETMPAKR

    FNLFLRRRLM FQKNQQSKDS IFFRDGIRQI DFVLSYVDDV KKDAELKAER

    RKEFETNLRK TGLELEIEDK RDSEDGRTYF VKIHAPWEVL VTYAEVLGIK

    MPIKESDIPR PKHTPISYVL GPVRLPLSVK YPHPEYFTAQ FSRHRQELFL

    IEDQATFFPS SSRNRIVYYI LSRCPFGIED GKKRFGIERL LNSNTYSSAY

    PLHDGQYWKP SEPPNPTNER YTLHQNWARF SYFYKEQPLD LIKNYYGEKI

    GIYFVFLGFY TEMLFFAAVV GLACFIYGLL SMEHNTSSTE ICDPEIGGQM

    IMCPLCDQVC DYWRLNSTCL ASKFSHLFDN ESTVFFAIFM GIWVTLFLEF

    WKQRQARLEY EWDLVDFEEE QQQLQLRPEF EAMCKHRKLN AVTKEMEPYM

    PLYTRIPWYF LSGATVTLWM SLVVTSMVAV IVYRLSVFAT FASFMESDAS

    LKQVKSFLTP QITTSLTGSC LNFIVILILN FFYEKISAWI TKMEIPRTYQ

    EYESSLTLKM FLFQFVNFYS SCFYVAFFKG KFVGYPGKYT YLFNEWRSEE

    CDPGGCLIEL TTQLTIIMTG KQIFGNIKEA IYPLALNWWR RRKARTNSEK

    LYSRWEQDHD LESFGPLGLF YEYLETVTQF GFVTLFVASF PLAPLLALIN

    NIVEIRVDAW KLTTQYRRTV ASKAHSIGVW QDILYGMAVL SVATNAFIVA

    FTSDIIPRLV YYYAYSTNAT QPMTGYVNNS LSVFLIADFP NHTAPSEKRD

    FITCRYRDYR YPPDDENKYF HNMQFWHVLA AKMTFIIVME HVVFLVKFLL

    AWMIPDVPKD VVERIKREKL MTIKILHDFE LNKLKENLGI NSNEFAKHVM

    IEENKAQLAK STL

  • UCSC uc001mqi.3; human.;




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