Mouse Akap1 ELISA Kit

Alternative name
Mouse dual specificity A-kinase-anchoring protein 1 ELISA Kit;Mouse D-AKAP-1 ELISA Kit;Mouse protein kinase A-anchoring protein 1 ELISA Kit;Mouse PRKA1 ELISA Kit;Mouse spermatid A-kinase anchor protein ELISA Kit;Mouse S-AKAP ELISA Kit;Mouse Akap ELISA Kit;Mouse AKAP121 ELISA Kit;Mouse AKAP84 ELISA Kit;Mouse C76494 ELISA Kit;Mouse C81186 ELISA Kit;Mouse DAKAP1 ELISA Kit;Mouse S-AKAP84 ELISA Kit;Mouse A kinase (PRKA) anchor protein 1 ELISA Kit;Mouse A-kinase anchor protein 1, mitochondrial ELISA Kit;Mouse a kinase anchor protein 1, mitochondrial ELISA Kit;
Catalog
E042292
species
Mouse
GeneAkap1
SpecificityThis assay has high sensitivity and excellent specificity for detection of AKAP1. No significant cross-reactivity or interference between AKAP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AKAP1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Akap1 ELISA Kit allows for the in vitro quantitative determination of Akap1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: AKAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AKAP1 antibody and an AKAP1-HRP conjugate. The assay sample and buffer are incubated together with AKAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AKAP1 concentration since AKAP1 from samples and AKAP1-HRP conjugate compete for the anti-AKAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AKAP1 from the sample, fewer sites are left to bind AKAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AKAP1 concentration in each sample is interpolated from this standard curve.
Mouse A-kinase anchor protein 1, mitochondrial Protein information
Uniprot ID AKAP1_MOUSE
Uniprot AC O08715; B1AR25; O08714; P97488;
UniGene Mm.2969;
GeneID 11640
KEGG mmu:11640;
Mouse A-kinase anchor protein 1, mitochondrial Protein SEQUENCE
SEQUENCE 857 AA; 92195 MW; 455679FCF95C28E6 CRC64;
MAIQLRSLFP LALPGMLALL GWWWFFSRKK DRLSSSDKQV ETLKVGPAIK
DRRLSEEACP GVLSVAPTVT QPPGREEQRC VDKPSTEPLA LPRTRQVRRR
SESSGNLPSV ADTRSQPGPC RDEIAKVELS LMGDKAKSIP LGCPLLPKDA
SFPYEAVERC KQESALGKTP GRGWPSPYAA SGEKARETGG TEGTGDAVLG
ENVSEEGLLS QECVSEVEKS EFPILAPGGG EGEEVSHGPP QVAELLKKEE
YIVGKLPSSF VEPVHSEPVK DEDALEPQVK GSSNTSDRDL AGELDKDETV
PENDQIKQAA FQLISQVILE ATEEFRATTV GKTVAQVHPT SATQPKGKEE
SCVPASQETS LGQDTSDPAS TRTGATASPS AEALPPKTYV SCLSSPLSGP
TKDQKPKNSA HHISLAPCPP PVTPQRQSLE GASNPRGDDN FVACMANNSQ
SVLSVSSLGQ CSDPVSTSGL EDSCTETISS SGDKAMTPPL PVSTQPFSNG
VLKEELSDLG TEDGWTMDTE ADHSGGSDGN SMDSVDSCCG LTKPDSPQSV
QAGSNPKKVD LIIWEIEVPK HLVGRLIGKQ GRYVSFLKQT SGAKIYISTL
PYTQNIQICH IEGSQHHVDK ALNLIGKKFK ELNLTNIYAP PLPSLALPSL
PMTSWLMLPD GITVEVIVVN QVNAGHLFVQ QHTHPTFHAL RSLDQQMYLC
YSQPGIPTLP TPVEITVICA APGADGAWWR AQVVASYEET NEVEIRYVDY
GGYKRVKVDV LRQIRSDFVT LPFQGAEVLL DSVVPLSDDD HFSPEADAAM
SEMTGNTALL AQVTSYSATG LPLIQLWSVV GDEVVLINRS LVERGLAQWV
DSYYASL
UCSC uc007kvu.2; mouse. [O08715-1];

Category

Mouse Akap1 ELISA Kit