Mouse Prkaa1 ELISA Kit

Alternative name
Mouse AMPK subunit alpha-1 ELISA Kit;Mouse acetyl-CoA carboxylase kinase ELISA Kit;Mouse ACACA kinase ELISA Kit;Mouse hydroxymethylglutaryl-CoA reductase kinase ELISA Kit;Mouse HMGCR kinase ELISA Kit;Mouse tau-protein kinase PRKAA1 ELISA Kit;Mouse AI194361 ELISA Kit;Mouse AI450832 ELISA Kit;Mouse AL024255 ELISA Kit;Mouse AMPKalpha1 ELISA Kit;Mouse C130083N04Rik ELISA Kit;Mouse protein kinase, AMP-activated, alpha 1 catalytic subunit ELISA Kit;Mouse 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit;Mouse AMP-activated protein kinase, alpha 1 catalytic subunit ELISA Kit;
Catalog
E064501
species
Mouse
GenePrkaa1
Other Species Human PRKAA1 ELISA Kit Porcine PRKAA1 ELISA Kit Rat Prkaa1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PRKAa1. No significant cross-reactivity or interference between Mouse PRKAa1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PRKAa1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Prkaa1 ELISA Kit allows for the in vitro quantitative determination of Prkaa1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRKAa1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRKAa1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PRKAa1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRKAa1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PRKAa1. You can calculate the concentration of Mouse PRKAa1 in the samples by comparing the OD of the samples to the standard curve.
Mouse 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein information
Uniprot ID AAPK1_MOUSE
Uniprot AC Q5EG47;
UniGene Mm.207004;
GeneID 105787
KEGG mmu:105787;
Mouse 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein SEQUENCE
SEQUENCE 559 AA; 63929 MW; 08632503663D395B CRC64;
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG
HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI
FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD
LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL
YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP
SVISLLKHML QVDPMKRAAI KDIREHEWFK QDLPKYLFPE DPSYSSTMID
DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD
FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ
GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP
VTSTFSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSISNY
RSCQRSDSDA EAQGKPSDVS LTSSVTSLDS SPVDVAPRPG SHTIEFFEMC
ANLIKILAQ
UCSC uc007vct.1; mouse.;

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Mouse Prkaa1 ELISA Kit