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Catalog
E023069
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species
Human
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GeneLung cancer marker
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Lung cancer marker. No significant cross-reactivity or interference between Lung cancer marker and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Lung cancer marker and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman Lung cancer marker ELISA Kit allows for the in vitro quantitative determination of Lung cancer marker , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCancer
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Product Description
specificalPrinciple of the assay: Lung cancer marker ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Lung cancer marker antibody and an Lung cancer marker-HRP conjugate. The assay sample and buffer are incubated together with Lung cancer marker-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Lung cancer marker concentration since Lung cancer marker from samples and Lung cancer marker-HRP conjugate compete for the anti-Lung cancer marker antibody binding site. Since the number of sites is limited, as more sites are occupied by Lung cancer marker from the sample, fewer sites are left to bind Lung cancer marker-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Lung cancer marker concentration in each sample is interpolated from this standard curve.
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