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Human DNAJB1 ELISA Kit

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  • Alternative name

    Human dnaJ protein homolog 1 ELISA Kit;Human heat shock 40 kDa protein 1 ELISA Kit;Human Hsp40 ELISA Kit;Human Heat shock protein 40 ELISA Kit;Human human DnaJ protein 1 ELISA Kit;Human hDj-1 ELISA Kit;Human DNAJ1 ELISA Kit;Human Hdj1 ELISA Kit;Human HSPF1 ELISA Kit;Human RSPH16B ELISA Kit;Human Sis1 ELISA Kit;Human DnaJ heat shock protein family (Hsp40) member B1 ELISA Kit;Human dnaJ homolog subfamily B member 1 ELISA Kit;Human DnaJ (Hsp40) homolog, subfamily B, member 1 ELISA Kit;Human radial spoke 16 homolog B ELISA Kit;
  • Catalog
    E012227
  • species
    Human
  • GeneDNAJB1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman DNAJB1 ELISA Kit allows for the in vitro quantitative determination of DNAJB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HSP-40 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HSP-40 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the HSP-40 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of HSP-40 can be calculated.
  • Human DnaJ homolog subfamily B member 1 Protein information
  • Uniprot ID DNJB1_HUMAN
  • Uniprot AC P25685; B4DX52;
  • UniGene Hs.515210;
  • GeneID 3337
  • KEGG hsa:3337;
  • Human DnaJ homolog subfamily B member 1 Protein SEQUENCE

  • SEQUENCE 340 AA; 38044 MW; 17545098B0C196DF CRC64;

    MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE

    AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA

    MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR

    SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE

    DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD

    GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE

    GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI

  • UCSC uc010xnr.2; human. [P25685-1];



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