SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BIRC5. No significant cross-reactivity or interference between Human BIRC5 and analogues was observed.
Intended UseHuman BIRC5 ELISA Kit allows for the in vitro quantitative determination of BIRC5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for API4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any API4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for API4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of API4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Baculoviral IAP repeat-containing protein 5 Protein information
Uniprot ID
BIRC5_HUMAN
Uniprot AC
Q75SP2; Q9P2W8;
UniGene
Hs.744872;
GeneID
332
KEGG
hsa:332;
Human Baculoviral IAP repeat-containing protein 5 Protein SEQUENCE