Human KIM-1 ELISA Kit

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  • Alternative name

    Hepatitis A virus cellular receptor 1 homolog ELISA KIT; Kidney injury molecule 1 ELISA KIT; KIM-1 ELISA KIT; T cell immunoglobulin and mucin domain-containing protein 1 ELISA KIT; TIMD-1Havcr1 ELISA KIT; Kim1 ELISA KIT; HAVcr-1 ELISA KIT; KIM-1 ELISA KIT; TIMD-1 ELISA KIT

  • Catalog
    E021795
  • species
    Human
  • GeneKIM-1
  • Other Species Mouse KIM-1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intended Uses For detection of KIM-1 in Human serum, plasma, urine, cell culture supernatan
  • Intended UseHuman KIM-1 ELISA Kit allows for the in vitro quantitative determination of KIM-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology; Research Assays; Assay Categories
  • Product Description
    specifical
    Principle of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-KIM-1 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-KIM-1 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the KIM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of KIM-1 can be calculated. Background: KIM1, also known as Hepatitis A virus cellular receptor 1, is a protein that in humans is encoded by the HAVCR1 gene, which maps to 5q33.2. It is a major cause of orally transmitted acute hepatitis, infects primate cells, but not dog or Human cells, after binding to the HAV cellular receptor (HAVCR). Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Khademi et al. (2004) found that differential expression of TIMs by Th1 and Th2 cells may be implicated in different phases of an autoimmune disease.



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