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Catalog
E002144
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species
Human
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GeneANG I
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Other Species
Human Ang-I ELISA KitHuman Ang-I ELISA KitHuman ANG- I R ELISA KitMouse Ang-I ELISA KitBovine Ang-I ELISA KitCanine Ang-I ELISA KitChicken Ang-I ELISA KitGeneral Ang-I ELISA KitPorcine Ang-I ELISA KitRabbit Ang-I ELISA KitRat Ang-I ELISA KitCamel Ang-I ELISA KitGoat Ang-I ELISA KitGuinea Pig Ang-I ELISA KitHamster Ang-I ELISA KitHorse Ang-I ELISA KitMonkey Ang-I ELISA KitSheep Ang-I ELISA Kit
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 75pg/ml
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Intended UseHuman ANG I ELISA Kit allows for the in vitro quantitative determination of ANG I , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ANG I antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ANG I antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ANG I amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ANG I can be calculated.
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