-
Alternative name
Human IL-8 ELISA Kit;Human C-X-C motif chemokine 8 ELISA Kit;Human chemokine (C-X-C motif) ligand 8 ELISA Kit;Human emoctakin ELISA Kit;Human granulocyte chemotactic protein 1 ELISA Kit;Human GCP-1 ELISA Kit;Human monocyte-derived neutrophil chemotactic factor ELISA Kit;Human MDNCF ELISA Kit;Human monocyte-derived neutrophil-activating peptide ELISA Kit;Human MONAP ELISA Kit;Human Neutrophil-activating protein 1 ELISA Kit;Human NAP-1 ELISA Kit;Human Protein 3-10C ELISA Kit;Human T-cell chemotactic factor ELISA Kit;Human GCP/IL-8 protein IV ELISA Kit;Human IL8/NAP1 form I ELISA Kit;Human (Ala-IL-8)77 ELISA Kit;Human GCP/IL-8 protein II ELISA Kit;Human IL-8(1-77) ELISA Kit;Human IL8/NAP1 form II ELISA Kit;Human MDNCF-b ELISA Kit;Human (Ser-IL-8)72 ELISA Kit;Human GCP/IL-8 protein I ELISA Kit;Human IL8/NAP1 form III ELISA Kit;Human Lymphocyte-derived neutrophil-activating factor ELISA Kit;Human LYNAP ELISA Kit;Human MDNCF-c ELISA Kit;Human Neutrophil-activating factor ELISA Kit;Human NAF ELISA Kit;Human GCP/IL-8 protein V ELISA Kit;Human IL8/NAP1 form IV ELISA Kit;Human GCP/IL-8 protein VI ELISA Kit;Human IL8/NAP1 form V ELISA Kit;Human GCP/IL-8 protein III ELISA Kit;Human IL8/NAP1 form VI ELISA Kit;Human IL8 ELISA Kit;Human GCP1 ELISA Kit;Human LECT ELISA Kit;Human LUCT ELISA Kit;Human NAP1 ELISA Kit;Human C-X-C motif chemokine ligand 8 ELISA Kit;Human interleukin-8 ELISA Kit;Human alveolar macrophage chemotactic factor I ELISA Kit;Human beta endothelial cell-derived neutrophil activating peptide ELISA Kit;Human beta-thromboglobulin-like protein ELISA Kit;Human interleukin 8 ELISA Kit;Human lung giant cell carcinoma-derived chemotactic protein ELISA Kit;Human lymphocyte derived neutrophil activating peptide ELISA Kit;Human neutrophil-activating peptide 1 ELISA Kit;Human small inducible cytokine subfamily B, member 8 ELISA Kit;Human tumor necrosis factor-induced gene 1 ELISA Kit;
-
Catalog
E019519
-
species
Human
-
GeneCXCL8
-
SpecificityNatural and recombinant human IL-8
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity< 1pg/ml
-
Intended UseHuman CXCL8 ELISA Kit allows for the in vitro quantitative determination of CXCL8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the assay: human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards(E.coli,S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-8 amount of sample captured in plate.
Background: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
-
Human IL-8(9-77) Protein information
-
Uniprot ID
IL8_HUMAN
-
Uniprot AC
Q9UCR9; Q9UCS0;
-
UniGene
Hs.624;
-
GeneID
3576
-
KEGG
hsa:3576;
-
Human IL-8(9-77) Protein SEQUENCE
-
SEQUENCE 99 AA; 11098 MW; 15C649996E89319F CRC64;
MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK
ELRVIESGPH
CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
-
UCSC
uc003hhe.3; human.;
-