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Alternative name
Interleukin-8 ELISA KIT; C-X-C motif chemokine 8 ELISA KIT; Chemokine (C-X-C motif) ligand 8 ELISA KIT; Emoctakin ELISA KIT; Granulocyte chemotactic protein 1 ELISA KIT; GCP-1CXCL8 ELISA KIT; IL8 ELISA KIT; IL-8 ELISA KIT; GCP-1 ELISA KIT; MDNCF ELISA KIT; MONAP ELISA KIT; NAP-1 ELISA KIT; LYNAP ELISA KIT; NAF ELISA KIT
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Catalog
E019518
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species
Human
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GeneIL8
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SpecificityThe Human IL-8 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-8 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity4 pg/ml.
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Intended UseHuman IL8 ELISA Kit allows for the in vitro quantitative determination of IL8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||The Human IL-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
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