Human IL24 ELISA Kit

Alternative name
Human IL-24 ELISA Kit;Human melanoma differentiation-associated gene 7 protein ELISA Kit;Human MDA-7 ELISA Kit;Human Suppression of tumorigenicity 16 protein ELISA Kit;Human MDA7 ELISA Kit;Human ST16 ELISA Kit;Human C49A ELISA Kit;Human FISP ELISA Kit;Human IL10B ELISA Kit;Human MOB5 ELISA Kit;Human interleukin 24 ELISA Kit;Human interleukin-24 ELISA Kit;Human IL-4-induced secreted protein ELISA Kit;Human melanocyte-associated Mda-7 ELISA Kit;Human suppression of tumorigenicity 16 (melanoma differentiation) ELISA Kit;
Catalog
E019444
species
Human
GeneIL24
SpecificityThe Human IL-24 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-24 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity30 pg/ml.
Intended UseHuman IL24 ELISA Kit allows for the in vitro quantitative determination of IL24 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-24 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-24 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-24 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin 24 (IL-24) is a cytokine belonging to the IL-10 family of cytokines that signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2. This interleukin is also known as Melanoma differentiation-associated 7 (mda-7) due to its discovery as a tumour suppressing protein. By radiation hybrid analysis, the MDA7 gene is mapped to 1q32.2-q41. IL-24 appears to control in cell survival and proliferation by inducing rapid activation of particular transcription factors called Stat-1 and Stat-3. Human IL24 was expressed as an approximately 35-kD protein that was converted to a 23-kD protein by deglycosylation. The IL24 protein shares 24 to 33% homology with other members of the IL10 family of cytokines, with highest homology with IL20. This cytokine is predominantly released by activated monocytes, macrophages and T helper 2 (Th2) cells and acts on non-haematopoietic tissues such as skin, lung and reproductive tissues. IL-24 performs important roles in wound healing, psoriasis and cancer.
Human Interleukin-24 Protein information
Uniprot ID IL24_HUMAN
Uniprot AC Q13007; Q2YHE5; Q53XZ7; Q5YLN8; Q96DB0; Q96KG4;
UniGene Hs.58831; Hs.744273;
GeneID 11009
KEGG hsa:11009;
Human Interleukin-24 Protein SEQUENCE
SEQUENCE 206 AA; 23825 MW; CB8135083EAB8DDD CRC64;
MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ
GQEFHFGPCQ VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV
SDAESCYLVH TLLEFYLKTV FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ
LQPSQENEMF SIRDSAHRRF LLFRRAFKQL DVEAALTKAL GEVDILLTWM
QKFYKL
UCSC uc001hes.3; human. [Q13007-1];

Human IL24 ELISA Kit

Contact Us