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Catalog
E019379
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species
Human
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GeneIL-17A/F elisa set
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SpecificityThe assay recognizes natural human IL-17 A/F. To define specificity of this IL-17 A/F antibody pair, several proteins were tested for cross reactivity using the IL-17 A/F pre-coated ELISA kit (which contains the same antibodies).There was no cross reactivity observed for any protein tested (IL-17A, IL-17B, IL-17D, IL-17E, IFN-gamma, Gal-1 and IL-33). The kit shows cross reactivity with the human IL17F.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe sensitivity, minimum detectable dose of this IL-17 A/F antibody pair was determined using the IL-17 A/F ELISA kit (which contains the same antibodies) and was found to be <6.6pg/ml. This was determined by adding 3 standard deviations to the mean OD ob
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Intended UseHuman IL-17A/F elisa set ELISA Sets allows for the in vitro quantitative determination of IL-17A/F elisa set , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyHuman ELISA Sets
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Product Description
specificalPrinciple of the assay: A capture Antibody highly specific for IL-17 A/F is coated to the wells a microtitre strip plate. Binding of IL- 17 A/F in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti-IL-17 A/F secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-17 A/F present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-17 A/F in any sample tested.
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