Human HSV 1 ELISA Kit

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  • Catalog
    E019007
  • species
    Human
  • GeneHSV 1
  • Other Species Human HSV 1/2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman HSV 1 ELISA Kit allows for the in vitro quantitative determination of HSV 1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyInfectious Disease; Immunology; Research Assays; Assay Categories
  • Product Description
    specifical
    Intended Use The MBS Herpes 1 IgA Antibody ELISA Test Kit has been designed for the determination of specific IgA antibodies against Herpes 1 in serum and plasma. For research use only, not for use in diagnostic procedures. PRINCIPLE OF THE TEST The MBS Herpes 1 IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). Herpes 1 antigen is bound on the surface of the microtiter strips. Diluted patient unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Herpes 1 antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.


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