Human GCH1 ELISA Kit

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  • Alternative name

    Human GTP cyclohydrolase I ELISA Kit;Human GTP-CH-I ELISA Kit;Human DYT5 ELISA Kit;Human GCH ELISA Kit;Human DYT14 ELISA Kit;Human DYT5a ELISA Kit;Human GTP-CH-1 ELISA Kit;Human GTPCH1 ELISA Kit;Human HPABH4B ELISA Kit;Human GTP cyclohydrolase 1 ELISA Kit;Human dystonia 14 ELISA Kit;Human guanosine 5'-triphosphate cyclohydrolase I ELISA Kit;

  • Catalog
    E017693
  • species
    Human
  • GeneGCH1
  • Standard CurveHuman GCH1 ELISA Kit
  • Other Species Mouse Gch1 ELISA KitRat Gch1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human GCH1. No significant cross-reactivity or interference between human GCH1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human GCH1 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman GCH1 ELISA Kit allows for the in vitro quantitative determination of GCH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GCH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human GTP cyclohydrolase 1 Protein information
  • Uniprot ID GCH1_HUMAN
  • Uniprot AC P30793; Q6FHY7; Q9Y4I8;
  • UniGene Hs.86724;
  • GeneID 2643
  • KEGG hsa:2643;
  • Human GTP cyclohydrolase 1 Protein SEQUENCE
  • SEQUENCE 250 AA; 27903 MW; B8A0CB344C598B9A CRC64;

    MEKGPVRAPA EKPRGARCSN GFPERDPPRP GPSRPAEKPP RPEAKSAQPA

    DGWKGERPRS EEDNELNLPN LAAAYSSILS SLGENPQRQG LLKTPWRAAS

    AMQFFTKGYQ ETISDVLNDA IFDEDHDEMV IVKDIDMFSM CEHHLVPFVG

    KVHIGYLPNK QVLGLSKLAR IVEIYSRRLQ VQERLTKQIA VAITEALRPA

    GVGVVVEATH MCMVMRGVQK MNSKTVTSTM LGVFREDPKT REEFLTLIRS

  • UCSC uc001xbh.2; human. [P30793-1];



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